| dc.description.abstract | The research work was conducted to assess the incidence of Salmonella and
Escherichia coli (E. coli) contamination in different fish farms and fish markets of
Dinajpur district of Bangladesh, and to identify the sources of contamination with
practical measures to reduce the level of contamination. The study was conducted for a
period of seven months from March, 2017 to September, 2017. Samples were collected
from eight Upazila of Dinajpur district at different fish farms and markets. Selected
Upazila were Dinajpur Sadar, Kaharol, Parbotipur, Birol, Fulbari, Bochagong,
Cirrirbondar, and Khansama. Two fish farms and fish markets were selected from each
Upazila. Isolation and identification of Salmonella and E. coli were done by the
conventional method that includes screening and biochemical tests. The results indicated
that all of the collected samples were contaminated with Salmonella and E. coli bacteria
both fish farms and fish markets. The level of incidence of Salmonella contamination
was greater in scum samples (93.8%) and for E. coli the higher contamination found in
water samples (81.3%) among fish farms samples. The study revealed that 87.5% water
and 57.8% fish samples of fish farms samples were Salmonella positive and about 46.3%
and 30.9% isolates were tested Salmonella positive for water and fish samples. On the
other hand, E. coli contamination was also high and about 75% of scum and 75.6% of
fish samples were positive whereas 24.8% and 41.6% isolates were tested E. coli positive
for scum and fish samples. In case of market samples, Salmonella contamination was
higher in basket samples (100%) and lower contamination found in fish samples (48.9%)
whereas 43.01% and 28.6% isolates were tested Salmonella positive for basket and fish
samples. Meanwhile, E. coli contamination for fish, basket, and mat samples were
75.6%, 68.8% and 75% respectively, and the isolates positive to E. coli were 35.8%,
21.52% and 36.4% for fish, basket and mat samples respectively. Fish farms condition
were not satisfactory in terms of flood, sewage pollution, used of animal manure, feed
and waste disposal, application of excessive fertilizer, lack of consciousness of farmers
about personal hygiene and sanitation, and in fish markets, stalls, parking, spacing,
sanitation, drainage and quality management systems were not improved. The results
was very much alarming. It is important to train up the farmers, fish handlers and fish
sellers about good aquaculture practice, HACCP system, fish transportation system, cool
chain management system, and overall quality management system of fishes. | en_US |
