| dc.description.abstract | Cirrhinus reba, one of the very popular minor carp species is distributed in the Indian subcontinent including Bangladesh. The distribution of this species has greatly reduced and has
become endemic in selected areas of Bangladesh. To conserve the species as well as for the
inclusion of aquaculture, attempts were taken by several researcher for its fingerling production
through induced breeding. To produce quality fish fingerling the genetic diversity needs to be
considered, the present study was aimed to assess the genetic variation of wild C. reba collected
from different regions of Bangladesh. Wild stocks of C. reba were collected from the rivers of
Dinajpur, Bogura, Jashore and Mymensingh regions. The muscle tissue samples of 10
individuals from each region were collected and preserved for DNA extraction. A total of 10
RAPD primers with GC content ranged from 60-70% were screened for single individuals of
each population. Out of ten primers screened, seven primers that gave amplified products were
screened again to check the reproducibility and stability of the bands. Finally, seven primers
which produced clear, reproducible, and produced both monomorphic and polymorphic bands
were selected to reveal the genetic variation of four wild populations of C. reba. The data were
analyzed using MS Excel, GenAlex, and PAST. The highest numbers of bands (12) in all the
C. reba populations were observed with primer OP E-15 and lowest (7) with primer OP E-05.
The fragment size detected with these seven primers ranged from 300-1000bp. The highest
(80%) and lowest (45%) percentage of polymorphic bands across the primers were observed
in the Jashore and the Bogura population. The highest pair-wise genetic variation were detected
in between Bogura and Dinajpur Population (0.223) and lowest in between Jashore and
Dinajpur Population (0.126). The highest gene flow (Nm=1.516) was found between the
Bogura and the Jashore populations, whereas the lowest value (Nm=0.467) was observed
between the Bogura and the Dinajpur populations. The UPGMA dendrogram based on genetic
distance indicated that the segregation of four population of C. reba into two clusters: the
Bogura population formed cluster I and three other population formed cluster II. The second
cluster was further sub-divided into two subgroups: the Mymensingh and Dinajpur population
formed sister group where Jashore was separated. | en_US |
