DETECTION OF NEWCASTLE DISEASE VIRUS BY RAPID NDV ANTIGEN TEST KIT METHOD AND ALSO DETECTION OF ANTIBODY TITER LEVEL PRE AND POST VACCINATION

Abstract

The present study was carried out for the detection of Newcastle disease virus (NDV) by rapid NDV antigen test kit method and also detection of antibody titer level from collected samples based on Anigen rapid NDV Ag test kit and serological test. The samples (oropharynx, spleen and kidney swab) were collected aseptically from different aged of live, sick and dead birds from different areas of Bogra district of Bangladesh. Among 20 samples, 8 were positive to NDV antigen. The total 40% samples were showed positive result. The sera samples were collected from six aged groups namely group-A, B, C, D, E & F of commercial broiler birds (pre and post vaccinated) from a small scale broiler farm in Dinajpur district of Bangladesh. Indirect ELISA (iELISA) was performed to detect the antibody titer level before and after vaccination with increasing of ages. In iELISA test showed that the highest antibody titer was recorded as 18295 in group-D at ages of 21* days post vaccination and lowest antibody titer was recorded as 417 in group F at ages of 35 days post vaccination. The highest mean titer was found in group-E as 9468 which at 35 days aged of birds and the lowest mean titer was found in group-A at 7 days of old birds as 6291 determined by a commercial ELISA kit (Biocheck, UK). Among the six age group of the same flock of small scale commercial broiler farm, 5 weeks aged broiler birds showed lower protective level (90%). The overall protective level was 98%.