• Login
    View Item 
    •   HSTUL IR
    • Faculty of Veterinary and Animal Science
    • Dept. of Microbiology
    • Masters Thesis
    • View Item
    •   HSTUL IR
    • Faculty of Veterinary and Animal Science
    • Dept. of Microbiology
    • Masters Thesis
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    DETECTION OF NEWCASTLE DISEASE VIRUS BY RAPID NDV ANTIGEN TEST KIT METHOD AND ALSO DETECTION OF ANTIBODY TITER LEVEL PRE AND POST VACCINATION

    Thumbnail
    View/Open
    MOST. AFSANA PARVIN REGISTRATION NO. 1305068 SESSION: 2013-2014 SEMESTER: JANUARY — JUNE, 2014 (19.43Mb)
    Date
    2014-06
    Author
    PARVIN, MOST. AFSANA
    Metadata
    Show full item record
    URI
    http://localhost:8080/xmlui/handle/123456789/838
    Collections
    • Masters Thesis
    Abstract
    The present study was carried out for the detection of Newcastle disease virus (NDV) by rapid NDV antigen test kit method and also detection of antibody titer level from collected samples based on Anigen rapid NDV Ag test kit and serological test. The samples (oropharynx, spleen and kidney swab) were collected aseptically from different aged of live, sick and dead birds from different areas of Bogra district of Bangladesh. Among 20 samples, 8 were positive to NDV antigen. The total 40% samples were showed positive result. The sera samples were collected from six aged groups namely group-A, B, C, D, E & F of commercial broiler birds (pre and post vaccinated) from a small scale broiler farm in Dinajpur district of Bangladesh. Indirect ELISA (iELISA) was performed to detect the antibody titer level before and after vaccination with increasing of ages. In iELISA test showed that the highest antibody titer was recorded as 18295 in group-D at ages of 21* days post vaccination and lowest antibody titer was recorded as 417 in group F at ages of 35 days post vaccination. The highest mean titer was found in group-E as 9468 which at 35 days aged of birds and the lowest mean titer was found in group-A at 7 days of old birds as 6291 determined by a commercial ELISA kit (Biocheck, UK). Among the six age group of the same flock of small scale commercial broiler farm, 5 weeks aged broiler birds showed lower protective level (90%). The overall protective level was 98%.

    Copyright ©  2022 Central Library, HSTU
    Contact Us | Send Feedback
    Customized by :Interlink Technologies Ltd.
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Copyright ©  2022 Central Library, HSTU
    Contact Us | Send Feedback
    Customized by :Interlink Technologies Ltd.