GENOTYPIC EFFECT AND GROWTH-REGULATORS ON MICROPROPAGATION OF BRINJAL

Abstract

The investigation was carried out in the Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period from September, 2011 to January, 2012 with a view to study the effect of genotypes and growth regulator on micropropagation of Brinjal (Solanum sp.). For this purpose, the three genotypes of Solanum viz. Protab, Green Ball and Ghemma Begun (Local) were used to assess their regeneration ability. Leaf segments of three genotypes of Solanum were cultured on MS medium with different concentrations and combinations of hormones. Callus induction ranged form was 66.00 to 82.67% among the genotypes, Protab showed early callusing (7.533 days) with maximum rate of callus induction (82.67%). The highest percentage of callusing appeared in MS + 3 mg/L 2, 4- D + 0.05 mg/L BAP considering all genotypes. The range of shoot regeneration recorded from 52.33% to 65.67% and Protab showed highest percentage of shoot regeneration (65.67%). Early and maximum rate of regeneration was found in MS + 1 mg/L NAA + 0.1 mg/L BAP for all the genotypes. The highest number of roots per shoot was counted in Protab (73.33%) on '/,>MS + 2 mg/L IBA + 0.4 mg/L BAP. Based on the overall performance, the genotype Protab appeared as the best for callus initiation, shoot and root formation and ultimately successful regeneration of plants.