ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF CLOSTRIDIUM PERFRINGENS FROM LAMB DYSENTERY

Abstract

This study was conducted for isolation, identification, and antibiogram study of Clostridium perfringens organism from lamb dysentery. Affected sheep showed clinical signs as dysentery, diarrhea, anorexia, weakness, and dehydration. Study was perform by using morpholigical, cultural, biochemical test and antibiogram study during the period from July to December 2012. A total of 20 faeces and cloacal swab were collected from affected sheep farm for this study out of 20 faeces and cloacal swab sample 5 were found to be positive. Samples were collected from different sheep farm of different area of Dinajpur District. Faeces and cloacal samples were collected aseptically and microbial examination was done by using Gram’s staining, cultural and biochemical techniques in the Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur. The percentages of positive sample were 25%, 40%, 12.5%, 33.33%, respectively in Rezanur sheep farm, Khalilur sheep farm, Hasan sheep farm and Mojammal sheep farm. Antibiotic sensitivity was also observed by observing the growth of organism on antibiotic sensitivity disc by discs diffusion method. The organisms showed highly sensitive against ciprofloxacin, livofloxacin, penicillin and moderately sensitive against amoxycilline, enrofloxacin, azithromycin, neomycin and resistant against gentamycin, streptomycin. Over all antibiotic sensitivity reaveled that penicillin, ciprofloxacin, livofloxacin were most efficacious followed by amoxycillin and streptomycin. So, it may be recommended that penicillin, livofloxacin and ciprofloxacin in optimum doses would resolve most cases of lamb dysentery.