IDENTIFICATION OF AVIAN LEUKOSIS VIRUS FROM LAYER CHICKEN BY SEROLOGICAL TEST AND EMBRYO INOCULATION TECHNIQUE

Abstract

The present experiment was assigned to identify the Avian Leukosis Virus as well as to know the prevalence of Avian Leukosis Virus in different age group of layer chickens. As per study design 92 cloacal swab were collected from chicken by previously sterilized cotton bud and the cloacal swab were harvested in eppendorf tube containing 1 ml of PBS and preserved at -20°C. Then the Avian Leukosis Virus determination was done by Direct Enzyme Linked Immuno Sorbent Assay and inoculation in embryonated chickens eggs. Out of the total 92 samples Avian Leukosis Virus positive, suspect and negative samples were 13, 3 and 76, respectively. Highest 5 positive samples found in grower chickens followed by 4 samples in brooding chicken and 4 samples in layer chickens. Suspect samples only 3 found in grower chickens. Percentages of positive samples were 14.5% and 13.3%% respectively in Sonali and Hy-line brown chickens where as 4.8% suspect samples found in Sonali chickens. This is may be due to the role of local type chickens that did not show clinical signs and might transmited infection to foreign chickens. This also might due to the failure of hatchery to maintain the hygienic and standard production procedure. The inoculation of cloacal swab was done into five 10 days old embryonated chicken eggs through yolk sac route. After 5 days of inoculation it was observed that death of the all embryos with haemorrhage. This result confirms the prevalence of Avian Leukosis Virus in the study area of Bangladesh. Avian Leukosis Virus transmitted horizontally and vertically and therefore measures should be taken to prevent spread of ALV are more demanding.