ISOLATION AND IDENTIFICATION OF ENTEROBACTERIA FROM APPARENTLY HEALTHY, SICK AND DEAD BROILERS

Abstract

The present research work was conducted with a view to isolation and identification of enterobacteria from apparently healthy, sick, and dead broilers. The sample were collected from Thakurgaon and Dinajpur district in Bangladesh and brought to the Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University(HSTU), Dinajpur, during the period from January to June, 2012. A total of 236 samples were collected from 6 flocks of 3 farms and the collected samples were liver, cloacal swab and intestinal swab from healthy, sick, and dead broilers respectively. The samples were composed of 95 healthy, 69 disease and 72 dead broilers and the isolated enterobacteria were Escherichia coli , and Salmonella spp. Out of 236 samples 185samples were positive for both Escherichia coli (115) and Salmonella spp.(70). The cultural prevalence of Escherichia coli in healthy broiler was 39.19 %, in sick broiler was 36.95 %, and in dead broiler was 58.14 %. The prevalence of Salmonella spp. was 17.40%, 23.14% and 26.69 % in healthy, sick and dead broilers respectively in this study. It was found that the highest prevalence of Escherichia coli and Salmonella spp. was 58.14 % and 26.69% respectively in case of dead broilers. It may be concluded from this study that the enteric bacteria usually remain in the gastrointestinal tract and as clinically over infection and do not produce clinical disease unless or until other factors are involved.