PATHOLOGICAL EVALUATION OF GUMBORO VACCINE PREPARED FROM FREEZE-DRIED CULTURE OF THE “LIVE GUMBORO VIRUS GM97 STRAIN” (HIPRA GUMBORO® - GM97) INCOMMERCIAL CHICKENS

Abstract

A modified form of Intermediate plus of Infectious Bursal Disease Vaccine (HIPRAGUMBORO~-GM97) prepared from the GM97 strain of Infectious Bursal Disease virus was tested for its pathogenicity in commercial chickens. A total of 500 unvaccinated Cobb-500 commercial chicks, raised in relative isolation from day old. 21 chicks were collected from experimental farm at Day: (D1), Diz, Dis, Diz, Dao, Dz3 and Dz. respectively three birds were collected from each respective day. Vaccine was administered at ocular route at Day1 and boosting was done at Dayi7 with drinking water. A typically affected flock was included in this study for the comparison of the study. Clinical signs were observed. All the sampled birds were subjected to detailed necropsy. The visible gross morbid lesions, Bursa/Body weight ratios was recorded. The bursae were collected, preserved at 10% formalin, processed, sectioned and stained for histopathology including determination of bursal lesion scores. Data's were analyzed statistically.