IN-VITRO THROMBOLYTIC AND ANTICOAGULANT ACTIVITY OF ETHANOLIC EXTRACT OF Carum copticum AND Triconella foenum-graecum

Abstract

An experiment was carried out in the laboratory of the department of physiology and pharmacology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, during the period of July-December/2014. Some plant and spices were collected from Dinajpur district. Primarily ethanol extract was prepared from those plant and spices. 20 micropipette was taken filled with 0.5 ml human blood and 3 micropipette was taken as excess micropipette for control. These two kind of micropipette were screened for assessment their comparative efficacy of clotted blood lysis. Ethanol extract was used at a dose of 100 micro ml/ micropipette. Then this micropipette was incubated at 37 degree centigrade temperature for Ihr 45 minutes. After incubation I found 4 plant and 2 spices has anticoagulant property. These plant are Fern, Arum, Cannabis Tezpata and spices are Ajawin and Pesta. Finally I was experimented with Ajawin and Methi in-vitro thrombolytic and anticoagulant property. Anticoagulant and thrombolytic property was experimented in laboratory compared with Streptokinase (SK). Streptokinase showed 100% efficacy after treatment. Efficacy was recorded by compare among streptokinase control treated extract. Ethanol ajawin extract lysis clotted blood 57.33%.