| dc.contributor.advisor | Farhana Sharmeen | |
| dc.contributor.author | ROY, TAPON KUMAR | |
| dc.date.accessioned | 2022-04-22T06:21:25Z | |
| dc.date.available | 2022-04-22T06:21:25Z | |
| dc.date.issued | 2010-08 | |
| dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/528 | |
| dc.description | Brassica is the most economically important genus in the Brassicaceae family formerly
known as Cruciferae under the order Rhoedale. It has an extensive diversity and
includes about 160 species mainly annual or biennial herbs. It is the third most important
edible oil source in the world after soybean and palm (FAO, 2003; Paizza and Folia,
2001; Walker and Both, 2001). This crop supplies about 13.2% of the world's edible oil
(Downey and Robbelen, 1989).
Several species and types of Brassica are oilseed crops, vegetable, forage crops, and
also used in the production of condiments, such as mustard. Brassica species are widely
used in the cuisine of many cultures and recognized as a valuable source of dietary
fiber. Brassica vegetables contain little fat and are sources of vitamins, minerals, and
fibre. They also contain a large number of novel phytochemicals, some of which
protect against carcinogenesis (Steinmetz and Potter, 1996). Hence Brassicas are
believed to be useful in the prevention of cancer. Forage Brassica is an important
source of animal feed in many countries. | en_US |
| dc.description.abstract | The experiment was carried out during the period from November 2009 to
February 2010, in the tissue culture laboratory of the Department of Genetics and
Plant Breeding, Hajee Mohammad Danesh Science and Technology University,
Dinajpur. Stem segments of five cultivars of Brassica spp. Viz. Daulat, Tori-7,
Safal, Rai-5 and BARI Sarisha-8 were used to observe their regeneration
potentialiy and to establish a suitable in vitro plantlet regeneration protocol of
Brassica spp. MS media supplemented with different phytohormone combinations
were used to observe the callus induction ability of the explants. The highest
percentage of callus induction (89.00%) was found in Tori-7 with the medium
supplemented with 1.5 mg/L BAP + 0.2 mg/L 2, 4-D + 3.0 mg/L AgNO3. The
lowest percentage of callus induction (50.00%) was found in Rai-5 on medium
supplemented with 3.0 mg/L BAP + 0.4 mg/L 2, 4-D + 3.0 mg/L AgNO.
Significant variations were observed among the cultivar and media composition
for shoot initiation and root formation. The cultivar Daulat was recorded to
produce highest percentage of shoot (65%) on medium supplemented with 1.0
mg/L BAP + 0.1 mg/L 2, 4-D + 3.0 mg/L AgNO3. On the contrary, Rai-5 on
medium supplemented with 3.0 mg/L BAP + 0.4 mg/L 2, 4-D + 3.0 mg/L AgNO;
showed minimum percentage of shoot regeneration (25.00%). The cultivar Daulat
was found to produce the highest percentage of root (80.00%) on 2 MS + 0.5
mg/L NAA. The cultivar Rai-5 in % MS medium with 0.5 mg/L NAA + 1.0 mg/L
IAA was observed to produce the lowest percentage of root. The regenerated
plantlets of Daulat with sufficient roots thus obtained were hardened and
transferred successfully to the pots and subsequently to field. Considering overall
performance the cultivar Daulat was found superior than the other cultivars. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR. | en_US |
| dc.subject | DETERMINATION OF AN IDEAL CULTIVAR | en_US |
| dc.title | IN VITRO REGENERATION OF MUSTARD (Brassica spp.) AND DETERMINATION OF AN IDEAL CULTIVAR | en_US |
| dc.type | Thesis | en_US |
