IN VITRO REGENERATION OF MUSTARD (Brassica spp.) AND DETERMINATION OF AN IDEAL CULTIVAR

Abstract

The experiment was carried out during the period from November 2009 to February 2010, in the tissue culture laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur. Stem segments of five cultivars of Brassica spp. Viz. Daulat, Tori-7, Safal, Rai-5 and BARI Sarisha-8 were used to observe their regeneration potentialiy and to establish a suitable in vitro plantlet regeneration protocol of Brassica spp. MS media supplemented with different phytohormone combinations were used to observe the callus induction ability of the explants. The highest percentage of callus induction (89.00%) was found in Tori-7 with the medium supplemented with 1.5 mg/L BAP + 0.2 mg/L 2, 4-D + 3.0 mg/L AgNO3. The lowest percentage of callus induction (50.00%) was found in Rai-5 on medium supplemented with 3.0 mg/L BAP + 0.4 mg/L 2, 4-D + 3.0 mg/L AgNO. Significant variations were observed among the cultivar and media composition for shoot initiation and root formation. The cultivar Daulat was recorded to produce highest percentage of shoot (65%) on medium supplemented with 1.0 mg/L BAP + 0.1 mg/L 2, 4-D + 3.0 mg/L AgNO3. On the contrary, Rai-5 on medium supplemented with 3.0 mg/L BAP + 0.4 mg/L 2, 4-D + 3.0 mg/L AgNO; showed minimum percentage of shoot regeneration (25.00%). The cultivar Daulat was found to produce the highest percentage of root (80.00%) on 2 MS + 0.5 mg/L NAA. The cultivar Rai-5 in % MS medium with 0.5 mg/L NAA + 1.0 mg/L IAA was observed to produce the lowest percentage of root. The regenerated plantlets of Daulat with sufficient roots thus obtained were hardened and transferred successfully to the pots and subsequently to field. Considering overall performance the cultivar Daulat was found superior than the other cultivars.