STUDY ON FLORAL BIOLOGY AND PLANT REGENERATION POTENTIAL IN MUNGBEAN (Vigna radiata L. Wilezek)

Abstract

The experiment was conducted at the experimental field and in the tissue culture laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur during July to November 2009. Four genotypes of mungbean (Vigna radiata L. Wilczek) BARI Mung-2, BARI Mung-4, BARI Mung-5 and BARI Mung-6 were used in these experiments. Flower biology was studied for days to first flowering, total number of pollens under 100x microscope, number of fertile pollen, number of sterile pollen, pollen fertility (%), pollen sterility (%) and yield/plant (g). The characters significantly varied in genotypes. BARI Mung 6 was the highest performing genotype for all the positive characters which enhance seed yield. Yield and yield attributes were assessed through correlation study. Yield was significantly correlated with number of fertile pollens and pollen fertility (%). Regeneration of mungbean was conducted with four genotypes of mungbean and different concentrations and combinations of hormones (2,4-D, NAA, BAP and IAA) were used to assess regeneration ability by using two explants (leaf and cotyledon). Regeneration potential was high from leaf explants for all genotypes. Highest percentage of callus was observed in hormonal combinations of MS+0.5mg/I 2,4- D, MS+0.5mg/1 2,4-d+0.5mg/1 BAP and MS+1.0mg/l 2,4-D+0.5mg/1 BAP with BARI Mung 6. A combined effect of Ts (MS+10.0mg/l Kn) with BARI Mung 6 showed highest performance in shoot regeneration. For root induction a combined effect of Ty (MS+ 0.1 mg/l IAA) with BARI Mung 6 showed highest performance. Among the four genotypes BARI Mung 6 was found to be effective for regeneration of mungbean.