CHARACTERIZATION AND ANTIBIOGRAM STUDY OF BACTERIAL PATHOGENS ISOLATED FROM OMPHALITIS IN CALVES

Abstract

The study was conducted for characterization and antibiogram study of bacterial pathogens isolated from Omphalitis in calves. The Study was performed by using staining, cultural, biochemical test and antibiogram study of identified filled isolates during the period of July’2012 to December’2012. A total of 21 pus samples were collected from Omphalitis affected calves. All the samples were collected from the different veterinary hospital under Rangpur district for this study. The positive samples were brought to the Microbiology laboratory of the Department of Microbiology, Faculty of Veterinary and animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur. A total of 21 pus samples from infected calves belonging to group-A (13), B (6) and C (2) and comprising 13, 6, 2 sample respectively were inoculated into different bacteriological media and stained by Grams staining technique. It was observed that the highest percentage of occurrence of Omphalitis obtained from group-A: (61.90%) followed by group-B: (28.55%) and group-C: (9.52%). It was observed that out of 21 isolates Staphylococcus species was 66.67% and E. coli was 33.33%. Overall sensitivity pattern of antibiotics to Omphalitis revealed that Levofloxacin, Penicillin, Ciprofloxacin and Azithromycin are most efficacious followed by Chloramphenicol and Doxycycline. Thus, it may be recommended that Ciprofloxacin, Penicillin and Levofloxacin in optimum doses would resolve most cases of Omphalitis in calves