PREVALENCE OF MYCOPLASMA GALLISEPTICUM ANTIBODIES IN CHICKEN AND ISOLATION OF SAME ORGANISM FROM THE SEROPOSITIVE CASES

Abstract

The prevalence of Mycoplasma gallisepticum (MG) antibody in chickens in 05 selected small scale layer Farms in Dinajpur district of Bangladesh was determined during the period March to August, 2010. To conduct this study a total of 304 (10% birds of farm) sera samples were collected. Rapid Serum Plate Agglutination (SPA) test was performed using commercial MG antigen to detect the presence of antibodies against MG. The over all prevalence of MG antibody was 54.6% in the study area. The highest prevalence (61.7) was found at March-April followed MayJune (50.8) and July-August (47.73). The result further revealed that the infection was higher (69.6%) in young birds than in adult birds (50.4%). It was also demonstrated that the infection was higher (68.5%) in large flocks than in small flocks (50.10%). M. gallisepticum was isolated from tracheal swabs obtained from seropositive birds by inoculating the tracheal swabs into Mycplasma agar and Mycoplasma broth media during the period of study.