| dc.description | Bangladesh is an agricultural country. Livestock is one of the important
sources of our national economy. Commercially milk production plays an
important role to alleviate poverty and to improve the economic status of the
people through self-employment. The total cattle population of the country is
about 24.50 million, which is about 1.79% of the world and 5.47% of Asian
cattle population (FAO, 2004). In the last 10 years the cattle population has
increased by 0.30% in contrast with 0.40% of the world. Number of cattle per
livestock household is 3.50 (Saadullah and Hossain, 2000) and that of 0.94
for all household (BBS, 2002). There is no statistics of cattle yet according to
type or variety in the country. Total milk production of cattle is 78200 MT
(SAIC 2003).There are about 24 million cows present in our Bangladesh
(DLS, 2008-09). The number of milking cow in Bangladesh is 3.75 million,
which is 35.00% of the total population of Bangladesh (DLS, 1998). The
annual milk production in Bangladesh is nearly 1.62 million metric ton which is
very low in respect of our demand which is nearly 9 million metric ton (DLS,
1998). Domestic and small cow farming have occupied important source of
income of our rural peoples. The domestic house holders, small farmers
however are facing a great problem with the diseases of udder of their
animals and this has become a threat to their economy. Inflammation of udder
or mastitis needs to be thoroughly studied with respect to the etiologic agents
and holistic approaches. | en_US |
| dc.description.abstract | The present study was conducted to isolate and identify Escherichia coli from the
cases of mastitis in cows at Gaibandha district of Bangladesh. For this, a total of
50 milk samples were collected from clinically & sub-clinically mastitis affected
and apparently healthy cows of different dairy farms and rural domestic holdings
at Gaibandha district in Bangladesh. The study was carried out during the period
from March’2010 to August’2011. The milk samples were collected and brought
to the Laboratory under Department of Microbiology, Faculty of Veterinary and
Animal Science, Hajee Mohammad Danesh Science and Technology University,
Dinajpur and subjected to different bacteriological tests for isolation and
identification of bacteria based on morphology, staining, cultural and biochemical
examinations. Antibiotic sensitivity of the isolated organisms was also studied.
The isolated bacteria were Escherichia coli 30.00 %, Staphylococcus spp. 20.00
%, Streptococcus spp. 20.00 %, Corynebacterium sp. 14.00 % and 16.00 Y%owere
unidentified gram positive & gram negative bacteria. On EMB agar Escherichia
coli showed greenish black colonies with metallic sheen, On nutrient broth
enough whitish turbidity, On MacConkey agar Bright pink colonies were formed.
In Gram’s staining technique the Escherichia coli revealed Gram negative, pink
color, short rod, cocci, coma, single, pair or in short chain arrangement. Sugar
fermentation tests (dextrose, lactose and maltose) were positive and the organism
produced acid & gas. In Hanging Drop slide method, the organisms were motile.
The organism in another biochemical test showed MR test positive, VP test
negative & Indole positive. Results of antibiotic sensitivity of Escherichia coli
showed that they were highly sensitive (+++) to Chloramphenicol (70.00 %),
Kanamycin (70.00 %) and Nitrofurantoin (90.00 %), moderately sensitive (++) to
Erythromycin (50.00 %), Less sensitive (+) to nalidaxic acid (35.00 %), Cephalexin
(35.00 %), Amoxycillin (35.00 %) and resistant to Cloxacilin, Ampicillin and
Cephradin. The present study dictates that the Escherichia coli was one of the
important agents responsible for the mastitis in dairy cows at Gaibandha district of
Bangladesh. | en_US |
