dc.contributor.author | RAHMAN, MD. KHALESUR | |
dc.date.accessioned | 2022-04-21T06:04:36Z | |
dc.date.available | 2022-04-21T06:04:36Z | |
dc.date.issued | 2012-12 | |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/386 | |
dc.description | Poultry farms in Bangladesh have witnessed a rapid growth in recent times with a
high population and income growth, urbanisation and high-income elasticity of
demand, the demand for poultry products is expected to increase appreciably in the
future. The poultry sector in Bangladesh is very important for the reduction of
poverty and creation of employment opportunities. Many people are directly
dependent on this industry for their livelihood. Poultry meat is the fastest growing
component of global meat production, consumption, and trade, with developing and
transition economies playing a leading role in its expansion. In addition to providing
opportunities to increased poultry exports, rising poultry production spurs growth in
global import demand for feeds and other inputs and in investment opportunities in
these sectors. Bangladesh now has a large and rapidly expanding poultry sector. At
present, poultry sector playing an important role to improve our national health
through supplying of animal protein. The annual per capita consumption of meat was
4.8 kg in 1985-86, which increased to 8.5 kg in 2004-05. On the other hand, in 1985-
86, the per capita consumption was 12 eggs annually, while this increased to 59 eggs
annually in 2004-05. This mammoth increase in the meat and egg consumption was
possible only for the exploration of poultry sector in Bangladesh. | en_US |
dc.description.abstract | Isolation and identification of infectious bursal disease virus (IBDV) from an
outbreak was based on clinico-postmortem investigation and serological test. The
clinical manifestations of the affected flocks were anorexia, depression, ruffled
feathers, diarrhoea and death. During postmortem examination swollen, soft and
edematous with yellowish discoloration bursa of fabricious was observed. Petechial
and ecchymotic haemorrhages were also observed in the leg and breast muscles.
Bursae of fabricius were collected aseptically and macerated seperately in
sterilized pestle and mortar to prepare a 10% (w/v) suspension in sterile PBS.
Inocultion of 10% bursal homogenate @ 100 ul through intranasal, intraocular and
intracloacal route of experimental birds (group-A and group-B). Group-C was kept as
uninoculated control. At day 3 post infection chickens belonging to group-A and
Group-B were showed clinical and postmortem lesion similar to that of naturally
infected birds. Morbidity was almost 100% and mortality was 40-60% due to
experimental infection. Experimental birds of Group-C were remaining normal. The
antibody titer of the experimentally infected chickens at day 28-post infection was
determined by indirect ELISA technique. At day 28 post inoculation chicken showed a
significant antibody response as in vaccinated birds. This indicated that birds were
infected by IBDV. 0.2ml of the 10% bursal suspension was inoculated on the CAM
route of 10 days old embryonated indigenous chicken eggs. Mortality of the one
embryo was observed as early as 48 hours post infection .While mortality of the other
embryos occurred between days 3 to 6 of inoculation. This indicated that IBDV causes
mortality in inoculated embryo through CAM route. | en_US |
dc.language.iso | en | en_US |
dc.publisher | HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR. | en_US |
dc.subject | Isolation and propagation of IBDV | en_US |
dc.subject | Materials required for postmortem examination | en_US |
dc.subject | Developing chicken embryos (DCE) | en_US |
dc.title | ISOLATION AND IDENTIFICATION OF INFECTIOUS BURSAL DISEASE VIRUS FROM AN OUTBREAK OF GUMBORO DISEASE AND PRODUCTION OF INFECTION IN EXPERIMENTAL BIRDS | en_US |
dc.type | Thesis | en_US |