Show simple item record

dc.contributor.authorRAHMAN, MD. KHALESUR
dc.date.accessioned2022-04-21T06:04:36Z
dc.date.available2022-04-21T06:04:36Z
dc.date.issued2012-12
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/386
dc.descriptionPoultry farms in Bangladesh have witnessed a rapid growth in recent times with a high population and income growth, urbanisation and high-income elasticity of demand, the demand for poultry products is expected to increase appreciably in the future. The poultry sector in Bangladesh is very important for the reduction of poverty and creation of employment opportunities. Many people are directly dependent on this industry for their livelihood. Poultry meat is the fastest growing component of global meat production, consumption, and trade, with developing and transition economies playing a leading role in its expansion. In addition to providing opportunities to increased poultry exports, rising poultry production spurs growth in global import demand for feeds and other inputs and in investment opportunities in these sectors. Bangladesh now has a large and rapidly expanding poultry sector. At present, poultry sector playing an important role to improve our national health through supplying of animal protein. The annual per capita consumption of meat was 4.8 kg in 1985-86, which increased to 8.5 kg in 2004-05. On the other hand, in 1985- 86, the per capita consumption was 12 eggs annually, while this increased to 59 eggs annually in 2004-05. This mammoth increase in the meat and egg consumption was possible only for the exploration of poultry sector in Bangladesh.en_US
dc.description.abstractIsolation and identification of infectious bursal disease virus (IBDV) from an outbreak was based on clinico-postmortem investigation and serological test. The clinical manifestations of the affected flocks were anorexia, depression, ruffled feathers, diarrhoea and death. During postmortem examination swollen, soft and edematous with yellowish discoloration bursa of fabricious was observed. Petechial and ecchymotic haemorrhages were also observed in the leg and breast muscles. Bursae of fabricius were collected aseptically and macerated seperately in sterilized pestle and mortar to prepare a 10% (w/v) suspension in sterile PBS. Inocultion of 10% bursal homogenate @ 100 ul through intranasal, intraocular and intracloacal route of experimental birds (group-A and group-B). Group-C was kept as uninoculated control. At day 3 post infection chickens belonging to group-A and Group-B were showed clinical and postmortem lesion similar to that of naturally infected birds. Morbidity was almost 100% and mortality was 40-60% due to experimental infection. Experimental birds of Group-C were remaining normal. The antibody titer of the experimentally infected chickens at day 28-post infection was determined by indirect ELISA technique. At day 28 post inoculation chicken showed a significant antibody response as in vaccinated birds. This indicated that birds were infected by IBDV. 0.2ml of the 10% bursal suspension was inoculated on the CAM route of 10 days old embryonated indigenous chicken eggs. Mortality of the one embryo was observed as early as 48 hours post infection .While mortality of the other embryos occurred between days 3 to 6 of inoculation. This indicated that IBDV causes mortality in inoculated embryo through CAM route.en_US
dc.language.isoenen_US
dc.publisherHAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR.en_US
dc.subjectIsolation and propagation of IBDVen_US
dc.subjectMaterials required for postmortem examinationen_US
dc.subjectDeveloping chicken embryos (DCE)en_US
dc.titleISOLATION AND IDENTIFICATION OF INFECTIOUS BURSAL DISEASE VIRUS FROM AN OUTBREAK OF GUMBORO DISEASE AND PRODUCTION OF INFECTION IN EXPERIMENTAL BIRDSen_US
dc.typeThesisen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record