| dc.description | Bangladesh is an agricultural country, which produces a good number of
oilseed crops like mustard, sesame, groundnut, linseed, niger, safflower, sunflower,
soybean and castor. The oleiferous Brassica represented by rapeseed and mustard
plays an important role in edible oil production of the world. It is the third most
important edible oil sources in the world after soybean and palm (FAO, 1998; Piazza
and Foglia, 2001; Walker and Boot, 2001). About 13.2% of the worlds edible oil
supply comes from this crop (Downey and Robbelen, 1989). In Bangladesh it tops
the list in respect of area and production among the oil crops grown in the country
(Mondo and Wahhab, 2001). The acreage productions of rape seed and mustard in
Bangladesh during 2007-2008 fiscal years are 431078 and 153588 M _ tons
respectively. The average yield of Brassica oilseed in Bangladesh is around
356kg/acre (BBS, 2008).Brassica spp. includes three groups namely (i) the mustard
(ii) the rapeseed and (iii) the cole crops. The component species of rapeseed are
Brassica campestris L., and Brassica napus L. While mustared group includes
Brassica Juncea L. Czern and Coss, Brassica nigra Koch and Brassica carinata
Brun (Yarnell, 1956). The varities under the apecies of Brassia campestris cultivated
in Bangladesh are Tori-7, BARI sarisha-8, BINA sarisha-3, BINA sarisha-4, NINA
sarisha-5, BARI sarisha-6 and those under Brassica juncea are Rai-5, Daulat,
Shambol, BARI sarisha-10 and BARI sarisha-11 (Modol and Wahhab, 2001;
Anonymous, 2004). The oleiferous Brassica spp. are the principal source of edible
oil in Bangladesh. Oils of plant origin are nutritionally superior to that of animal
origin (Singh, 2001). Oils and fats are concentrated source of calories. The essential
fatty acids content in oils and fats play an important role in several metabolic
processes (Kaul and Das, 1986). Vegetable oils are used mostly for edible purposes
and a significant portion in used in industrially. | en_US |
| dc.description.abstract | The investigation was carried out in the Tissue Culture Laboratory of the
Department of Genetics and Plant Breeding, Hajee Mohammad Danesh
Science and Technology University, Dinajpur during November ,2008 to
May, 2009 with a view to study in vitro regeneration of Brassica spp.
from leaf explant, For this purpose, the four genotypes of Brassica viz.
BARI Sarisha-9, BARI Sarisha-11, Tori-7 and Sampad were used to'
assess their regeneration ability.
Leaf segments of the four genotypes of Brassica were cultured on MS
medium with different concentrations and combinations of hormones.
The range of percent callus induction was 55-100%. Among the four
genotypes, BARI Sarisha-9 genotype showed early callusing (6.22 days)
with maximum rate of callus induction (96.11%). Early and maximum
rate of callusing appeared in MS + 1.5 mg/L 2, 4-D + 0.3 mg/L NAA
+ 2.0 mg/L AgNO; for all genotypes.
Shoot regeneration ranged from 50% to 78.33% and BARI Sarisha-9 had the
highest percentage of shoot regeneration (72.22%). Early and maximum
rate of regeneration was found in MS + 3.5 mg/L BAP + 0.5 mg/L
NAA + 2.0 mg/L AgNO; for all the genotypes. The highest number of
roots per shoot was counted in BARI Sarisha-9 (76.67%), in "/yMS +
0.5 mg/L NAA. Considering the overall performance, genotype BARI
Sarisha-9 appeared the best for callus formation, shoot regeneration and root
formation. | en_US |
