| dc.description.abstract | A study was conducted with leaf explants of chilli for regeneration on MS medium
supplemented with different concentrations and combination of auxins and cytokinins.
For callus induction, five different concentrations and combinations of growth regulators
viz. MS + 2.0 mg/L Kinetin +3.0 mg/L 2,4-D, MS+ 1.5 mg/L Kinetin +2.5 mg/L 2,4-D,
MS+1.0 mg/L Kinetin +2.0 mg/L 2,4-D, MS+ 0.5 mg/L Kinetin+ 1.5 mg/L 2,4-D and
MS +0.05 mg/L Kinetin +1.0 mg/L 2,4-D were used. The highest percentage of callus(
91.67%) was obtained in MS medium supplemented with 2.0mg/L Kinetin + 3.0 mg/L
2,4-D 7.00 days. Among the three varieties, Sian) hot showed the best performance on
callus induction. On the other hand, leaf induced shoot bud in different combinations of
MS medium supplemented with different combinations like MS+3.0 mg/L IAA+ 1.0
mg/L BAP, MS+ 3.0 mg/L IAA +2.0 mg/L BAP, MS + 1.5 mg/L IAA +2.5 mg/L BAP,
MS +1.0 mg/L IAA +3.5 mg/L BAP and MS+ 1.0 mg/L IAA+ 5.0 mg/L BAP. Leaf
explants showed the best performance in MS+3.0 mg/L IAA+1.0 mg/L BAP in terms of
percent shoot regeneration (71.67%) and number of callus showing shoot (14.33). The
shoot bud showed the best performance in MS+ 3.0 mg/L IAA+1.0 mg/L BAP
combination and Siam hot showed the remarkable performance on shoot regeneration.
On the other hand, best root regeneration (80.00%) was found on MS+3.0 mg/L IBA+1.0
mg/L BAP combination and the genotype Siam hot showed the best performance on root
regeneration. | en_US |
