MICROBIOLGGICAL QUALITY OF COMMERCIALLY — AVAILABLE POULTRY FEEDS SOLD IN BANGLADESH

Abstract

The present study was conducted to identify the organisms in poultry feed manufactured by different poultry feed producing company namely Paragon, Aftab, Index and Jomjom sold in retail stores in Bangladesh. The samples were analyzed to determine the microbiological quality of poultry feed and like total viable count, presence of gram positive and gram negative pathogenic bacteria and fungi and as well as the effect of these organism in poultry. Four feed samples of each company were collected from different dealer of Rangpur city. The bacteriological analyses were done at the Microbiology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur. During January to june — 2014. Total viable count (TVC) was performed according to the American Public Health Association, using plate count agar medium for TVC, Eosine methylene blue (EMB) agar media for total E. coli count and salmonella-Shigella agar for total salmonella count. Biochemical properties of the isolates were also studied and reaction in TSI agar slant was observed. MIU test, MR test, VP test etc. were also performed. Sabourauds Dextrose agar media were also used for detection of presence of fungus. The virulence effect of the organism present in feed were observed by inoculating the organism in poultry. The average TVC for Aftab is 3.28 x 10°CFU/gm (log 5.5) was lower than Paragon is 5.45 x 10° CFU/gm (log 6.7) and average TVC for Jomjom 4.5 x 10° CFU/gm(log 5.7) was lower than Index 5.14 x 10°CFU/gm (log 6.7). The presence of fungi could be as a result of their high pathogenecity as reported ty researchers elsewhere. These organisms can causes several poultry and farm animal infections, they also produce mycotoxins that are also of public health importance to both humans and their farmed animals. The average EF. coli counts obtained from the study was in Aftab 8.26 x 10° (log 3.8), in Paragon 6.25 x 10° (log 5.7), in Jomjom 5.65 x 10° (log 4.7), in Index 5.5 x 10° (log 5.8). The presence of high numbers of E. coli in was little bit high indicate the poor hygienic practices during manufacture, post process contamination and unsatisfactory transportation and Preservation. Statistically the E. coli were more closely related to total viable counts than the Salmonella counts in the samples of Aftab 2.68 x 10° CFU/gm (log 3.4), Paragon 3.15x 10* CFU/gm (log 4.5), Jomjom 4.46 x 10° CFU/gm (log 3.6) and Index 1.19 x 10° CFU/gm (log 4.0) were respectively.