ISOLATION AND IDENTIFICATION OF MYCOPLASMA GALLISEPTICUM FROM LAYER BIRDS AT THE NORTHERN PART OF BANGLADESH

Abstract

Isolation and Identification of Mycoplsma gallisepticum (MG) from layer chicken from the selected small scale farms of northern part of Bangladesh was determined during the period of January to June, 2013. Isolation and Identification of Mycoplsma gallisepticum (MG) based on clinico-postmortem investigation, serological test and culture method. The clinical manifestation of the affected flocks were principally with a respiratory disease, like tracheal infections, there was rattling sounds, nasal discharge, and coughing and in a more severe illness increased mortality associated with infection of the lungs and airsacs. During the postmortem examination excess mucus or catarrhal exudates in nasal and paranasal passages specially in trachea, bronchi and massive air sacculitis was observed. To conduct the serological test a total of 320 sera samples were collected and Rapid Serum Plate Agglutination Test (RSPT) was performed by using the commercial MG antigen to detect the presence of antibodies against the MG. The overall prevalence of MG antibody was 43.93% in the study area. The higher prevalence (48.69%) was found on winter season than summer season (33.33%). The study also revealed that the infection was higher (69.69%) in the early laying stage (18-20 weeks) than older (61-65 weeks) age (22.30%). M. gallisepticum was isolated from tracheal swabs obtained from seropositive birds by inoculating the tracheal swabs into Mycoplasma agar media which produce overall 82.21% characteristics specific colony.