| dc.contributor.advisor | Md. Hasanuzzaman | |
| dc.contributor.author | ADHIKERY, BIBEKANANDA | |
| dc.date.accessioned | 2022-04-19T09:01:46Z | |
| dc.date.available | 2022-04-19T09:01:46Z | |
| dc.date.issued | 2009-06 | |
| dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/244 | |
| dc.description | Chillies are the fruits or berries of plants belonging to the genus Capsicum of the
Nightshade family, Solanaceae. The genus Capsicum consists of about 25 wild and 5
domesticated species. The five domesticated species are Capsicum annuum L.,
Capsicum frutescens L., Capsicum chinense Jacq., Capsicum baccatum L., and
Capsicum pubescens R & P. (IBPGR, 1983). Of the domesticated species, Capsicum
annuum is the most economically important and includes both mild and pungent fruit
types and native to tropical and temperate regions of the American. (Hunziker, 1979;
Pickersgill, 1991; Esbhaugh, 1993; Heiser, 1995.) | en_US |
| dc.description.abstract | A study was conducted with cotyledon of chilli as explants for regeneration on MS
medium supplemented with different concentrations of auxins and cytokines. For the
callus induction, three different combinations of growth regulators viz. 0.05 mg/L
kinetin + 1.5 mg/L 2, 4-D, 0.5 mg/L kinetin + 2.0 mg/L 2, 4-D and 1.0 mg/L kinetin +
3.0 mg/L 2, 4-D were used. The highest percentage of callus (96.29%) was obtained
in MS medium supplemented with 1.0 mg/L kinetin+3.0 mg/L 2, 4-D within 12.66
days. Among three genotypes, C1013 showed the best performance on callus
induction. On the other hand, cotyledon induced shoot bud in different combinations
of MS medium supplemented with different combinations like 0.1 mg/L IAA+5.0
mg/L BAP, 1.0 mg/L IAA+2.0 mg/L BAP and 0.1 mg/L IAA+ 0.1 mg/L BAP.
Cotyledon showed the best performance in 1.0 mg/L IAA+2.0 mg/L BAP in terms
of percent shoot regeneration (92.59%) and number of callus showing shoot (16.66).
The shoot bud showed the best performance in MS+1.0 mg/L IAA+2.0 mg/L BAP
combination and C1013 also showed the remarkable performance on shoot
regeneration. On the other hand, root regeneration (86.66%) was found on MS+3.0
mg/L IBA +1.0 mg/L BAP combination and C1011 showed the best performance on
root regeneration. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | HAJEE MOHAMMOD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR | en_US |
| dc.subject | Sterilization of Culture Room and Transfer Area | en_US |
| dc.subject | Sterilization of Experimental Materials (Seeds) | en_US |
| dc.subject | Culture Methods | en_US |
| dc.subject | Subculture of Callus for Shoot Regenerations | en_US |
| dc.title | IN VITRO PLANT REGENERATION OF CHILLI (Capsicum spp.) USING COTYLEDON EXPLANT | en_US |
| dc.type | Thesis | en_US |
