IN-VITRO THROMBOLYTIC ACTIVITY OF ETHANOLIC EXTRACT OF SOME TRADITIONAL PLANTS

Abstract

Atherothrombosis or thrombosis is a major cause of global life threatening heart and cerebral diseases For the Treatment myocardial infarction, many thrombolytic agents are used. Among them, streptokinase is remarkable and widely used. But It is noted that all available thrombolytic agents still have significant deficiencies, including the necessity of large doses to be maximally effective, limited fibrin specificity and a significant associated bleeding tendency and high cost. Herbal agent or thrombolytic plants can solve those problems. Consider this, present study was designed to investigate thrombolytic activity of some traditional plants. Some medicinal plants and spices were collected from Dinajpur district. Primarily ethanol extract was prepared from those plant and spices. 12 micro pipettes were taken filled with human blood and 6 micropipettes were taken as excess micropipette for positive and negative control. These two kinds of micropipette were screened for assessment their comparative efficacy of clotted blood lysis. Ethanol extract was use data dose of 100 micro ml/micro pipette. Then this micro pipette was incubated at 37 degree centigrade temperature for 90 minutes. After incubation I found 2 plants and 2 spices have thrombolytic activity. These plants are Blumea lacera (Kukurmutha), Persicaria hydropiper (Bishkatali) and spices are Trigonellla foenum (methi) and Carum copticum (Ajawin). Finally, I experimented those plants in laboratory compared with Streptokinase (SK) as positive control and water as negative control. Streptokinase showed 75.18% efficacy after treatment water showed 2.28% efficacy after treatment Efficacy was recorded by compare among streptokinase control treated extract. Ethanol extract of Ajawin, methi, Kukurmutha and Bishkatali lysis clotted blood 57.25%, 41.31%, 45.00%, 35.52% respectively.In those result show that the efficacy of ajawan extract is more efficient than other extracts.