EFFECT OF GENOTYPES AND GROWTH-REGULATORS ON MICROPROPAGATION OF BRINJAL (solanum sp.)

Abstract

The investigation was carried out in the Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period from November, 2009 to April, 2010 with a view to study the effect of genotypes and growth regulator on micropropagation of Brinjal (Solanum sp.). For this purpose, the three genotypes of Solanum viz. Chomok, Long Black and Jhumky were used to assess their regeneration ability. Leaf segments of three genotypes of Solanum were cultured on MS medium with different concentrations and combinations of hormones. Callus induction ranged form was 66.00 to 82.67% among the genotypes, Chomok showed early callusing (7.533 days) with maximum rate of callus induction (82.67%). The highest percentage of callusing appeared in MS + 3 mg/L 2, 4-D + 0.05 mg/L BAP considering all genotypes. The range of shoot regeneration recorded from 52.33% to 65.67% and Chomok showed highest percentage of shoot regeneration (65.67%). Early and maximum rate of regeneration was found in MS + 1 mg/L NAA + 0.1 mg/L BAP for all the genotypes. The highest number of roots per shoot was counted in Chomok (73.33%) on ‘MS + 2 mg/L IBA + 0.4 mg/L BAP. Based on the overall performance, the genotype Chomok appeared as the best for callus initiation, shoot and root formation and ultimately successful regeneration of plants.