| dc.description.abstract | The investigation was carried out in the Tissue Culture Laboratory of the
Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science
and Technology University, Dinajpur during November, 2009 to April, 2010 with
a view to study in vitro regeneration of Lentil (Lens culinaris Medik) from leaf
discs and nodal segments explants. For this purpose, three varieties of Lentil viz.
BARI Masur -3, BARI Masur -4 and BARI Masur -5 were used to assess their
regeneration ability. Leaf disc and nodal segments of the three genotypes of Lentil
were cultured on MS medium with different concentrations and combinations of
growth regulators. Among the three varieties, BARI Masur -4 showed early
callusing (17.67 days) with maximum rate of callus induction (83.33%) incase of
leaf disc culture and also showed highest percentage of callus induction (80.55%)
with minimum days (17.33 days) with nodal segment as_ explant. Early and
maximum rate of callusing appeared in T, (MS +1.5 mg/L 2, 4-D + 0.5 mg/L
BAP) and T; (MS +2 mg/L 2, 4-D + 0.5 mg/L NAA) from leaf disc and nodal
segment respectively. BARI Masur -4 had the highest percentage of shoot
regeneration from leaf disc (69.44%) and nodal segment (66.66%) explant. Early
and maximum rate of regeneration was found in T, (MS+2.0 mg/L Kn + 0.2 mg/L
NAA) and T3 (MS medium +0.5 mg/L BAP+ 0.25 mg/L Kn). The highest number
of roots per shoot was counted in BARI Masur -4 (47.23%), in T; (MS medium
containing 10mg/L IAA) and T, (MS medium containing 15mg/L IBA)
considering leaf disc and nodal segment as explant. Considering the overall
performance, genotype BARI Masur -4 appeared the best for callus formation,
shoot regeneration and root formation. | en_US |
