ISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM RAW CHICKEN MEAT IN DINAJPUR DISTRICTISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM RAW CHICKEN MEAT IN DINAJPUR DISTRICT

Abstract

The present research work was under taken to isolate and identify the associated bacteria of chicken raw meat and to find out the effective antibiotics against the bacteria through antibiogram studies in Dinajpur district Bangladesh. July to November, 2019. A total of 48 samples were randomly collected from markets and transported to the Microbiology laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur. After processing of samples primary culture was done in nutrient broth and nutrient agar then pure culture was obtained from different selective media. The level of bacterial load were significantly higher the first sampling in comparison with the second and third one respectively. The prevalence of Escherichia coli, Salmonella spp. and Staphylococcus spp. were 47.9%, 39.6% and 12.5% respectively. All pure isolates were subjected to Antibiogram assay test by disc diffusion method against 8 different antibiotics. E. coli isolates were sensitive moxifloxacin. Highest resistant to chloraphenicol, tetracycline and streptomycin. Among all Salmonella spp., isolates, chloraphenicol showed the highest susceptibility pattern followed by the tetracycline, azithromycin, ampicillin and Erythromycin. Highest resistant pattern was showed by penicillin, Cefalexin and amoxicillin. In case of Staphylococcus spp., chloraphenicol showed the highest susceptibility pattern followed by the streptomycin and streptomycin found sensitive in this study. Highest resistant pattern showed by the tetracycline, azithromycin, ampicillin and tetracycline. This study revealed that broiler meat sold at some local markets in Dinajpur city were contaminated with multiple species of multidrug resistant bacteria which may risk for human health.