DETEMINATION OF MICROBIAL QUALITY OF DRINKING WATER OBTAINED FROM DIFFERENT SOURCES FOR HUMAN, POULTRY AND DAIRY FARM IN DINAJPUR DISTRICT OF BANGLADESH

Abstract

Total ninety (90) samples, thirty 30 from human tubewell water, 30 water samples of dairy farm and 30 water samples of poultry farm were collected and tested for TVC and MPN to to determine the microbial quality of drinking water. The mean HPC of human tubewell water were found 2.55×103 , 3.11×103 , 2.85×103 , 2.88×103 and 3.20×103 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest HPC was found in Chirirbandar 3.20×103 CFU mL-1 and lowest found in Sadar 2.55×103 CFU mL-1. The MPN values were found 2.16 , 2.16 , 2.50 , 1.83 and 2.33 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest MPN value was found in Kaharol 2.50 and lowest were found in sadar 2.16 and Chirirbandar 2.16 coliforms/100 ml .The mean HPC of dairy farm tubewell water were found 2.3×103 , 3.6×103 , 2.7×103 , 3.2×103 and 3.4×103 CFU mL-1 in Sadar, Birgonj, Kaharol ,Birol and Chirirbandar .The highest HPC of dairy farm tubewell water was found in Birgonj 3.6×103 CFU mL-1 and lowest HPC was found in Sadar 2.3×103 CFU mL-1. The MPN values were found 1.66, 2.33, 2.66, 2.00 and 2.66 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The highest MPN values were found in Kaharol 2.66 coliforms/100 ml and Chirirbandar 2.66 coliforms /100 ml and lowest was found in sadar 1.66 coliforms/100 ml of water.The mean of HPC of dairy farm manger water were in 4.66×107 , 2.0×107 , l 2.8×107 , 2.8×107 and 4.2×107 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest mean HPC of dairy farm manger water was found in Sadar 4.66×107 CFU mL-1 and lowest found in Birgonj 2.0×107 CFU mL-1 .The MPN values were found 14.00, 17.33, 14.00, 16.33 and 15.33 coliforms /100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The MPN of dairy farm manger water was highest in Birgonj 17.33 coliforms /100 ml and lowest were found in Sadar 14.00 coliforms /100 ml and Kaharol 14.00 coliforms/100 ml.The geometric mean of HPC of Poultry farm (Tubewel/Machine) water were Sadar 2.8×103 CFU mL-1 , Birgonj 2.67×103 CFU mL-1 , Kaharol 3.0×103 CFU mL-1 , Birol 2.57×103 CFU mL-1 and Chirirbandar 3.0×103 CFU mL-1 .In this study, it was found that HPC of Poultry farm tubewell water was highest in Kaharol 3.0×103 CFU mL-1 and lowest in Birol 2.57×103 CFU mL-1 .The MPN values of Poultry farm tubewell water were found 2.66, 2.00, 2.33, 1.66 and 2.00 coliforms/100 ml of drinking water in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The MPN of Poultry farm tubewell water was highest in Sadar 2.66 coliforms/100 ml and lowest in Birol 1.66 coliforms/100 ml.The mean of HPC of Poultry farm waterer water were found 4.2×107 , 2.00×107 , 3.25×107 , 1.00×107 and 2.00×107 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. Highest HPC of Poultry farm waterer water was found in Sadar 4.2×107 CFU mL-1 and lowest in Birol 1.00×107 CFU mL-1 . The MPN values of poultry waterer water were 12.33, 9.66, 10.33, 13.33 and 12.33 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The highest MPN value was found in Birol 13.33 coliforms/100 ml and lowest were found in Birol 9.66 coliforms/100 ml. From ninety water samples seven bacteria were isolated among them the most frequent isolate was Escherichia coli 82(15.95%) followed by Salmonella spp 81(15.75%), Shigella spp 80(15.56%), Klebsiella spp 79(15.36%), Vibrio spp 73(14.20%), Pseudomonas spp 60(11.67%) and Staphylococcus spp 59(11.47%). The antimicrobial susceptibility pattern showed that the isolates were highly resistant to ampicillin, amoxicillin, erythromycin and chloramphenicol and susceptible to gentamicin, azithromycin, colistin, ceftriaxone, levofloxacin and ciprofloxacin. Out of seven bacteria two bacteria (Escherichia coli and Vibrio spp) are subjected to amplified by using 16S rRNA gene based PCR