EXPLORING OF CHLORPYRIFOS AND DIAZINON MINERALIZING ENDOPHYTIC AND SOIL BACTERIA ASSOCIATED WITH PLANT GROWTH PROMOTION TRAITS TO PROMOTE SUSTAINABLE DEVELOPMENT

Abstract

The present investigation was carried out to exploit the chlorpyrifos, and diazinon mineralizing endophytic and soil bacteria associated with plant growth promotion traits to promote sustainable development. To carry out this research total twenty individual bacteria were isolated from Aman rice soil, tomato root and cauliflower root which were grown on pesticide (chlorpyrifos, and diazinon) containing normal growth media where pesticides were used as sole carbon sources. These isolates were named as D-1 soil, D-2 soil, D-3 soil, D-4 soil, D-5 soil, CS-1 soil, CS-2 soil, CS-3 soil, CS-4 soil, CS-5 soil, D-T.R.1, D-T.R.2, D-T.R.3, CS-T.R.1, CS-T.R.2, CS-T.R.3, D-C.R.1, D-C.R.2, CS-C.R.1,and CS-C.R.2 according to their sources. On the basis of biochemical characterization of eighteen bacterial isolates except D-5 soil and CS-T.R.3, 12% for Methyl Red test, 45% for Vogus Proskauer (VP) test, 100% for catalase test, 28% for oxidase test, 100% for TSI, CIU, Sucrose, Dextrose and MIU test, 67% for lactose and 95% for Urease and maltose were positive. All the isolates had the different cell wall degrading enzymes (cellulase, xylanase, protease and amylase) producing capability. The sixteen (16) isolates among 20 were characterized by 16s rRNA gene sequencing. All the isolates showed positive result on growth promoting traits experiment. The maximum Indole-3 acetic acid (IAA) production was observed in CS-T.R.2, maximum ACC deaminase activity in CS-3 soil where all the isolates showed positive result in nitrogen fixing and phosphate solubilizing test. Further confirmation of growth promotion activity, these isolates were applied on eggplant. The significant (ρ≤0.05) result was found on CS-5 soil and CS-T.R.1 treated eggplant’s root length and CS-1 soil and CS2 soil treated eggplant’s shoot length which were higher than control one. This experiment was examined on petri dishes after treated the eggplant seed with the isolates in the dark. During pot experiment, the significant (ρ≤0.05) result was found on CS-2 soil, CS-T.R.2, D-1 soil and D-2 soil bacteria treated eggplant in root length and shoot length elongation along with consortium treatment of bacteria had higher effects on eggplant growth. The pesticides bio-degradation was confirmed by FT-IR analysis. D-T.R.1and D-4 soil had the high capability of degrading the P=S bond and C=C bond of diazinon respectively. CS-1 soil, CS-2 soil and CS-5 soil bacteria had the high capability of degrading the C-Cl bond and the benzene special vibration bond of chlorpyrifos. These strains have plant growth promoting traits which was examined on eggplant associated with pesticide degradation which cover the three goals (life on earth, life below water, and Good health) to promote sustainable development.