PROXIMATE COMPOSITION ANALYSIS OF Leonurus sibiricus AND PREDICTION OF ITS BIOACTIVE COMPOUND-PROTEIN INTERACTIONS IN Homo sapiens

Abstract

The experiment was conducted in the research Laboratory of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh, during the period January, 2018 to March, 2018 to find out the analysis of the Proximate composition of L. sibiricus, a herbaceous medicinal plant and the possible effects of bioactive compounds of Rutin and Quercetin. There is no information about the proximate composition of L. siribicus. We collected the sample from two different districts Dinajpur and Nilphamari to observe either it has any significant differences of proximate composition or not. In the proximate composition analysis, the Ash, Starch, Fiber, Fat, Protein, Non-reducing sugar (Sucrose), Invert (Total) sugar, Reducing sugar contents of L. sibiricus in Dinajpur were found, 12.83±0.942%, 1.122±0.045 mg/100g, 1.122±0.045%, 4.950±0.062%, 19.73±1.616%, 2.150±0.224 mg/100g, 3.393±0.258 mg/100g, 1.246±0.050 mg/100g, respectively, while in the L. sibiricus in Nilphamari these compositions were found 15.92±0.286%, 2.134±0.043 mg/100g, 22.90±0.145%, 4.790±0.474%, 26.77±0.631%, 1.832±0.153 mg/100g, 4.203±0.136 mg/100g, 2.371±0.048 mg/100g respectively. The Data revealed that proximate compositions, mineral contents and vitamins can be varied depending on the growing area, soil and environmental factors. In addition that, we used some bioinformatics tools to analyze the possible effects of bioactive compounds of L. sibiricus. For this, we selected two most important bioactive compounds Rutin and Quercetin to know the interactions in Homo sapiens. Because, we have future plan to use L. siribicus to control the blood sugar in diabetic patients. Rutin and Quercetin are the two bioactive compounds which can control or reduce the blood sugar of diabetic patients. We have applied some recently developed computational tools in this present study. Our results showed that Rutin and Quercetin have either some positive or negative interactions with 10 proteins and subsequently these 10 proteins have been found to interact with 99 other proteins through the application of bioinformatics tools. All of these proteins are involved in cellular, molecular and biological functions in human body.