EFFECT OF EXTRACTION PARAMETERS ON THE YIELD AND QUALITY OF PECTIN FROM MANGO (Mangifera indica L.) PEELS
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Abstract
Pectin is a structural heteropolysaccharide, commonly found in the peels of various fruits
and vegetables that are used as a gelling agent and stabilizer in food and pharmaceutical
industries. The present study was focused on the potential of mango peel as a source of
pectin. Therefore, pectin was extracted from mango (mangifera Indica) peels powder
using HCl as extractant at five different extraction conditions viz. temperatures (60, 70,
80, 90 and 100°C), pH (1.0, 1.5, 2.0, 2.5 and 3.0) and time (60, 80, 100, 120 and 140
minutes). Pectin yields varied from 14.60% to 28.42% depending on extraction
conditions used in this study. The highest yield (28.06%) condition was found at 90°C
temperature, 1.5 pH (28.42%) and 120 minutes (28.06%) of extraction time. The quality
parameters of pectin in terms of equivalent weight (450.45g to 1324.24g), methoxyl
content (5.15 to 7.90%), anhydrouronic acid content (48.93 to 74.62%) and degree of
esterification (43.28% to 77.14%) was found to be in satisfactory levels. On the contrary,
soluble sugar content (12.43% to 23.49%) was slightly higher compared to the existing
data for various pectin sources, because matured and ripen mangoes were used in this
extraction procedure. In some conditions e.g. at higher pH (e.g. 3.0), lower time (e.g 60
minutes) and low temperature (e.g 60°C) the anhydrouronic acid (AUA) content was
moderately low compared to the standard data of Food Chemicals Codex, 1996. This is
an indication of the existence of protein in the extracted pectin. The pectin extracted
from mango peel powder also applied in jelly formulation at different concentration i.e
0.5%, 1.0%, and 1.5% to see the quality characteristics of jelly like jelly grade and
especially the sensory characteristics. From the sensory evaluation of jelly it was found
that the jelly prepared with 1.0% of extracted mango pectin was more excellent and more
acceptable than other pectin concentration. The colour, taste, appearance, flavor and
overall acceptability was highly acceptable at 1.0% pectin concentration compared to
other percentage of pectin used in the formulation of jelly. On the other hand, the result
obtained for commercial or standard pectin at 0.5% concentration was moderately good
more acceptable than other pectin concentration. Through this study, manufacturer will
get valuable information regarding the quality of mango peel pectin. Conclusively,
mango peel can be used as new source of pectin for application in different food and
pharmaceutical products.