| dc.description.abstract | Prawns sold in local market could be microbiologically spoiled
intrinsically or extrinsically. The research study was attempted to
detect Total Bacterial Count (TBC), Total Coliform Counts (TCC),
Total Salmonella-Shigella (TSS) Counts and Total Vibrio Counts
(TVC). The abundance of bacterial loads was determined from thirty
prawn samples. The density of total bacteria was detected in all the
prawn samples were significantly higher (p< 0.001). Mean±SEM of
TBC, TCC, TSS and TVC of prawn samples was 4.49±0.24, 5.20±0.29,
4.60±0.30 and 5.59±0.21 log10 CFU/g respectively. The total
bacterial counts (TBC) ranged between 3.8×102 CFU/g to 2.8×106
CFU/g. The total coliforms counts (TCC) ranged between 4.9×102
CFU/g to 3.52×107 CFU/g. Furthermore, total Salmonella-Shigella
(TSS) counts ranged from 1.65×102 CFU/g to 1.3×107 CFU/g. The
total Vibrio counts (TVC) of thirty samples were ranged between
4.0×103 CFU/g to 2.5×107 CFU/g. The highest Mean±SEM of TBC,
TCC, TSS and TVC were found in Gopalgong bazaar (4.76±0.39 log10
CFU/g), Rail gate bazaar (6.13±0.41 log10 CFU/g), Rail gate bazaar
(5.79±0.32 log10 CFU/g) and Rail gate bazaar (5.29±0.40 log10
CFU/g) respectively. But the bacterial load found in prawns did not
vary significantly among the three selected local markets of Dinajpur
city. A total of thirty prawn samples were examined. All of them were
found to be contaminated with different microbes characterized as
follows- E. coli (100%), Salmonella spp. (100%), Vibrio spp. (66.7%),
Klebsiella spp. (40%) and Shigella spp. (33.3%). Isolated bacteria
were identified using the biochemical tests namely Indole test, Methyl
red test, Voges - Proskauer test, Citrate utilization test, Triple sugar
vi
Iron test and MIU test. The results revealed that the bacterial load
found to be higher in most of the samples. The study of antibiotic
resistance pattern showed a number of pathogenic isolates to be
drug-resistant. All of the isolates were found to be 100% resistant
against Tetracycline. E. coli and Vibrio spp. also showed 100%
resistant against Cefixime and Erythromycin respectively.
Key words- Prawn, Total Bacterial Count (TBC), Total Coliform Count
(TCC), Total Salmonella-Shigella (TSS) Count, Total Vibrio Counts
(TVC), Antibiotic resistance. | en_US |
