ISOLATION AND IDENTIFICATION OF BACTERIA FROM DIFFERENT STREET FOOD AND STUDY OF THEIR ANTIBIOGRAM

Abstract

This study was carried out to investigate the microbiological quality of different street food (Amra, Fusca, Chanachur and Guava) sold by various street vendor at Dinajpur, Bangladesh. A total of 20 samples were collected randomly from street vendors in Dinajpur and tested for the presence of bacteria following conventional microbiological processes. Among 20 Amra, Fusca, Chanachur and Guava food samples, all (100%) had bacterial contamination. The total viable count (TVC) in diferrent street food samples was ranged from 8.0 × 105 CFU/g to 6.7x107 CFU/g. Among the 20 samples Escherichia coli 7(35%), Staphylococcus spp 6(30%), Klebsiella spp 5(25%), Salmonella spp 1(5%) and Shigella spp 1 (5%) were isolated and identified from the samples. In 20 street food samples Escherichia coli were found highest 3(50%) Amra samples and 2(40%) Guava samples, then another organisms Staphylococcus spp was found highest 2(50%) Chanachur and 2(40%) Fusca samples and then Klebsiella spp was found highest 2(40%) Fusca samples. Antibiotic sensitivity test showed that Shigella spp, Staphylococcus spp, Klebsiella spp, Salmonella spp and Escherichia coli were sensitive to Ciprofloxacin. Staphylococcus spp, Klebsiella spp, Escherichia coli were sensitive to Gentamycin and found Salmonella spp resistance to Gentamycin. Klebsiella spp and Staphylococcus spp were sensitive to Neomycin and found Shigella spp were resistance to Neomycin. Klebsiella spp and Shigella spp were intermediate sensitive to Kenamycin. All isolates found resistant to Cefixime, Cefalexin, Erythromycin, Fusidic acid, Cefuroxime and Aztreonam. This study indicated that the different street foods showed wide range of TVC which exceed acceptance level. According to the microbiological standard of foods in Bangladesh, aerobic plate counts ranges from 10 to 102 CFU/ g can be said to be safe, 102 to < 104 CFU/g acceptable, 104 to <105 CFU/g not acceptable and =105 CFU/g can be said to be extremely hazardous for public health. All of the sample harbor multidrug resistant food borne bacteria which might cause public health hazards if these antibiotic resistance transfer to human. Hence, it is recommended that a more close supervision of such food type should be carried out by relevant authorities to avoid any future pathogen outbreaks.