| dc.description.abstract | The study was conducted for identification of pathogenic bacteria from Thai koi (Anabas
testudineus) and an experimental infection test was run for finding out the actual
causative agent of the infection. Due to perform the experiment, the fish sample was
collected from a fish farm located in Fulbari, Dinajpur to the Microbiology lab of the
University campus and placed in tray for taking sample from different infected part such
as gill, slime, muscle, fin of the fish by using wire loop then these were taking in the
nutrient ager medium for observing the culture of bacteria. After then specific culture
media , Salmonella-Shigella media, Mannitol salt agar media, Mac-Conkey (MaC) agar
media and Eosin Methylene Blue media were used for observing specific bacterial
characteristichs. Then biochemical tests, Methyl red (MR), Voges-proskaure test, Triple
sugar iron test, Indole test were performed for bacterial identification. As a result
Salmonella spp., Shigella spp., Klebshiella spp. and Staphylococcus spp. were confirmed.
Catalase test and Simon citrate test was also performed. Then Grams staining method was
followed for microscopic observation of identified bacteria. Then experimental infection
test was performed in Aquaculture lab by setting up 5 aquarium holding fresh fish. The
fresh water and identified bacteria were added specifically to the aquarium and it was
continued for 15 days for observing infectious symptoms. After 15 days the fish with
Salmonella spp. and Staphylococcus spp. were showed infectious symptoms but other did
not any change in physical appearance. So it can be said that Staphylococcus spp. and
Salmonella spp. are able to show ulcerative symptoms in Thai koi (Anabas testudineus)
that is a bacterial infection. | en_US |
