| dc.description.abstract | Newcastle disease is highly contagious disease of poultry and causes huge economic loss in
Bangladesh. Regardless of vaccination, the disease caused considerable mortality in all types
of poultry. In the present study, the efficacy of different vaccination programs against
Newcastle disease virus was performed to examine duration and level of antibody in layer
farm with different diluents. The experiment was carried out in Dinajpur district and
serological test was carried out in the Department of Microbiology, Faculty of Veterinary and
Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur
and AG Agro Poultry Lab, Gazipur during the period of July, 2017 to December, 2017. For
this, a total of 200 blood samples were randomly collected from different age groups of layer
birds which were vaccinated with commercially available two live and one killed vaccine
namely AVI ND LaSota, CEVAC NEW L and ITA-New (ND). Pre-vaccination serum
antibody titre (Mean ± SD) of Group a birds were 5.97±0.75. At first, birds were vaccinated
with Avi ND LASOTA® vaccine, and CEVAC® NEW L vaccine. MASTERBLUE powder
and ADVANCE NON-FAT were used as diluents in vaccination. After 40 days of postvaccination, sera samples were procured and showed serum antibody titre (Mean ± SD) 7.0 ±
0.93. In case of Farm-B, before vaccination antibody titers levels against NDV were Mean ±
SD 5.85±0.75. Birds of Farm-B were vaccinated with Avi ND LaSota® vaccine. After 40
days of post-vaccination, titre were Mean ± SD were 6.7 ± 0.93. Then birds were vaccinated
with ITA-New (ND) ® which were killed vaccine. After 40 days of post-vaccination, the titer
were Mean ± SD 8.15± 0.93. MASTERBLUE powder and ADVANCE NON-FAT is a
suitable diluents to be used in vaccination against Newcastle disease because tap water may
inactivate the vaccine due to its impurity in addition to containing higher level of chlorine
and even the type of pipes or vessels used to distribute the drinking water. The results showed
that the level of protection of vaccinated birds were satisfactory. | en_US |
