Determination of Immune Response against Newcastle Disease Virus in Layer Chicken Vaccinated with Different Types of Vaccine and Diluents

Abstract

Newcastle disease is highly contagious disease of poultry and causes huge economic loss in Bangladesh. Regardless of vaccination, the disease caused considerable mortality in all types of poultry. In the present study, the efficacy of different vaccination programs against Newcastle disease virus was performed to examine duration and level of antibody in layer farm with different diluents. The experiment was carried out in Dinajpur district and serological test was carried out in the Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur and AG Agro Poultry Lab, Gazipur during the period of July, 2017 to December, 2017. For this, a total of 200 blood samples were randomly collected from different age groups of layer birds which were vaccinated with commercially available two live and one killed vaccine namely AVI ND LaSota, CEVAC NEW L and ITA-New (ND). Pre-vaccination serum antibody titre (Mean ± SD) of Group a birds were 5.97±0.75. At first, birds were vaccinated with Avi ND LASOTA® vaccine, and CEVAC® NEW L vaccine. MASTERBLUE powder and ADVANCE NON-FAT were used as diluents in vaccination. After 40 days of postvaccination, sera samples were procured and showed serum antibody titre (Mean ± SD) 7.0 ± 0.93. In case of Farm-B, before vaccination antibody titers levels against NDV were Mean ± SD 5.85±0.75. Birds of Farm-B were vaccinated with Avi ND LaSota® vaccine. After 40 days of post-vaccination, titre were Mean ± SD were 6.7 ± 0.93. Then birds were vaccinated with ITA-New (ND) ® which were killed vaccine. After 40 days of post-vaccination, the titer were Mean ± SD 8.15± 0.93. MASTERBLUE powder and ADVANCE NON-FAT is a suitable diluents to be used in vaccination against Newcastle disease because tap water may inactivate the vaccine due to its impurity in addition to containing higher level of chlorine and even the type of pipes or vessels used to distribute the drinking water. The results showed that the level of protection of vaccinated birds were satisfactory.