ISOLATION AND IDENTIFICATION OF ORAL BACTERIA CAUSING PERIODONTITIS IN HUMAN WITH ANTIBIOGRAM STUDY
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Abstract
The Presence study was conducted to isolate and identify the oral bacteria causing
periodontitis in human by using morphological, cultural and biochemical techniques.
However the prevalence of periodontitis was higher in young (65%) in comparing
with child(1.3%) and adult(29%) stages. The prevalence of oral bacteria was higher in
plaque in comperison with caries (27%) and tartar (23%) respectively. On the other
hand the prevalence was also varied with the sugar consumption. In which the highest
prevalence were absence in high sugar intake patients (30%) and lowest in less sugar
intake patients (25.3%). Out of 150 samples 85% samples were found to be positive
for Streptococcus and 75% for Staphylococcus and 65% for Porphyromonus
gingivalis by using morphological and biochemical techniques. The identified isolates
were also subjected to antibiogram study. In which most of the organism were
resistant to maximum antibiotic which are available in commercially and sensitive to
Ofloxacine. In this study it was observed that most of the oral bacteria were resistant
to commonly used antibiotics that is the related for treating the oral infection on the
other hand ofloxacine was only the sensitive antibiotic against oral bacteria. From this
study it was concluded that determination of antibiotic susceptibility is very important
in the determination of first line of choice for drugs in clinical control of periodontitis.