| dc.description.abstract | The present study was conducted to isolate and identify Escherichia
coli from raw milk at Rangpur division of Bangladesh. For this, a total
of 60 milk samples were collected from different dairy farms and
rural domestic holdings at Rangpur division in Bangladesh. The study
was carried out during the period from July, 2017- May, 2018. The
milk samples were collected and brought to the Laboratory under
Department of Microbiology, Faculty of Veterinary and Animal
Science, Hajee Mohammad Danesh Science and Technology
University, Dinajpur and subjected to different bacteriological tests
for isolation and identification of bacteria based on morphology,
staining, cultural and biochemical examinations. Molecular test using
PCR was also studied. The isolated bacteria were Escherichia coli
30.00%, Staphycoccus spp. 20%, Salmonella spp 20%,
Corynebacterium spp. 16%. On nutrient broth enough whitish
turbidity, On MacConkey agar Bright pink colonies were formed and
On EMB agar Escherichia coil showed greenish black colonies with
metallic sheen. In Gram’s staining technique the Escherichia coil
revealed Gram negative, pink color, short rod, cocci, coma, single,
pair or in short chain arrangement. The organism in biochemical test
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showed MR test positive, VP test negative & Indole positive. In
statistical analysis, the prevalence of the isolates in different dairy
farm found from this study was non-significant. Present study
indicates that the Escherichia coli was one of the important bacteria
that available in raw milk of the sample collection area at Rangpur
district of Bangladesh. | en_US |
