CHARACTERIZATION OF STREPTOCOCCUS PNEUMONIAE STRAIN ISOLATED FROM PNEUMONIA CASES OF CHILD PATIENTS

Abstract

Streptococcus pneumoniae is a leading cause of global morbidity and mortality in children causing pneumonia. The increasing incidence of antibiotic-resistant S. pneumoniae strains worldwide poses a threat to the effective treatment of these infections. For this purpose, the present research was conducted during the period of January– November 2018. The samples were collected from three main hospitals of Dinajpur district of Bangladesh. A total of 40 samples were randomly collected & analyzed through different bacteriological, biochemical, moleculer & antibiotic susceptibility testing. Out of 40 samples, positive cases of pneumonia were found to be 37.5% & 15 isolates were isolated. The Frequency of pneumonia in relation to age were 3-5yrs (50%), 6-8yrs (33.33%), 9- 11yrs (25%) & 12-15 (20%). Distribution of organism based on age difference were 3-5 years (7%), 6-8 years (20%), 9-11 years (6.67%) & 12-15 years (30%). The present study reveals that the study area had no significant effect (P > 0.05), but age (P < 0.05) & socio-economic status (P < 0.05) had significant effects on the prevalence of pneumonia in pneumonia patients. In study area, the highest prevalence was found in M Abdur Rahim Medical College Hospital (40%), followed by Arobindo Shishu Hospital (33.33%) & Islami Bank Community Hospital (40%) respectively. Among the age group, the prevalence of pneumonia was highest (50%) in 3-5 years age group, then 33.33% in 12-15 years age group, 25% in 6-8 years age group & 20% in 9-11 years age group. Highest prevalence of pneumonia was found in poor socio-economic status (54.54%), followed by medium (16.66%) & rich (16.66%) socio-economic condition respectively. Streptococcus pneumoniae was characterized by 16S rRNA Sequencing & the identified strain was Streptococcus pneumoniae NBRC102642. The antibiotic study revealed that all of the isolates of Streptococcus pneumoniae were resistant to most of the drugs, but found sensitive to Neomycin, Kanamycin & Streptomycin followed by Erythromycin, Azithromycin & Bacitracin. Avoidence of indiscriminate use of antibiotics & development of nationwide awareness can play an important role for the prevention of pneumonia.