ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF ENTEROVIRULENT ESCHERICHIA COLI RECOVERED FROM DIARRHOEIC GOAT

Abstract

Enterovirulent Escherichia coli remain as an important etiological agent of goat diarrhoea in Bangladesh. The present study was designed for the cultural and biochemical characterization of Goat E. coli from diarrhoeic and apparently healthy goat in Rangpur district for the period from January to July, 2012. Out of 135 faecal samples, 85 from diarrhoeic and 50 from apparently healthy goat collected from different areas in Rangpur district. It was found that the prevalence of E. coli was higher in diarrhoeic goats 18.82 % (16) and non diarrhoeic 14.00 % (07) were found to be positive for E. coli. The cultural characterization of all positive goats E. coli revealed greenish black colony with metallic sheen in Eosine methylene blue agar, bright pink color smooth transparent colony in MacConkey agar and slight pinkish smooth colony in Salmonella-Shigella agar. In case of biochemical characterization, all of the isolates showed fermentation of dextrose, sucrose and maltose with the production of acid and gas, negative result to Voges-Proskaure test, positive result to Methylred test and differential result to Indole test. The antibiotic sensitivity and resistance pattern showed that the isolates of goat E. coli were highly sensitive to ciprofloxacin and gentamicin while moderately sensitive to colistin, livofloxacin and azithromycin and less sensitive to ceftraexon and tetracyclin and resistant to amoxycillin, ampicillin, erythromycin, and neomycin.