ISOLATION AND IDENTIFICATION OF BACTERIA FROM DIFFERENT TYPES OF TABLE EGGS AND EGG STORING TRAYS COLLECTED FROM LOCAL MARKET

Abstract

Egg and egg products are vital human dietary content due to lower cost. But improperly handled egg could be source of public health hazard. The present study was aimed to isolate and identify the egg-borne bacteria from egg storing trays & different parts of table eggs such as egg shell and yolk. The anti-biogram profile of the isolated bacteria was also assessed. A total of 90 samples comprising different types of table eggs and egg storing trays were collected randomly from different grocery shops of Dinajpur sadar during the period of January to June, 2018. All the microbiological study were confirmed by cultural and biochemical test. Antibiotic sensitivity test of the bacterial isolates was performed by disc diffusion method. Out of 90 samples, the number of bacteria isolated were Escherichia coli 17 (18.89%), Salmonella spp. 10 (11.11%), Pseudomonas spp. 22 (24.44%), Klebsiella spp. 14 (15.55%) and Staphylococcus spp. 35 (38.89%). In the present study, highest contamination found in egg shell and egg storing trays than yolk. It was also observed that out of five (05) isolated pathogens Staphylococcus spp. (33.98%) was more abundant in the table eggs in comparison with E. coli (16.50%), Pseudomonas spp. (21.36%), Klebsiella spp. (13.59%) and Salmonella spp. (14.56%). The antibiotic study revealed that isolated E. coli were resistant to most of the antibiotics used for the sensitivity test .Other isolates, Staphylococcus spp. and Salmonella spp. were resistant to Amoxicillin, Erythromycin and Cephalexin. Pseudomonas spp. and Klebsiella spp. were resistant to Cephalexin, Tetracycline and Kanamycin. This may be due to indiscriminate use of antibiotics in the treatment of bacterial disease in the layer house. The table eggs harbor multi-drug resistant bacteria may impose public health hazards if these bacteria are transferred to human through food chain. Eggs are exposed to contamination due to faulty sanitary condition during production to storage, high temperature, dust, hand touching, and all other surrounding pollution state.