| dc.description.abstract | Seven genotypes of wheat including four released varieties were used in embryo culture to assess
the totipotency of the tested genotypes. The N6 medium was used for callus induction green spots
initiation. Four growth characters like, callus growth rate, relative fresh weight growth, relative
growth rate and callus induction rate and five embryogenic characters e.g. embryo size (mm),
number of embryos inoculated, number of calli induced, number of calli with green spots and
green shooting were measured. The study was conducted from October, 2017 to July, 2018. The
experiment was conducted at Plant Tissue Culture Laboratory under the Department of Genetics
and Plant Breeding. A 10 days embryo was placed on nutrient medium. Each genotype was
containing 9 embryos. In significant test, all the parameters related callus induction found
statistically significant among the seven genotypes. Three genotypes, like Satyn-17, Kanchan
and Satyn-24 did not produce any green shoot; hence, these genotypes were not suitable for
embryo culture. On the contrary, out of four released wheat varieties, BARI Gom-25 produced
the highest green shoot (83%), which was followed by BARI gom-29 (71%). Therefore these
two varieties might be incorporated in distant hybridization to get desirable genetic
recombination through hybrid recue program through embryo culture. Correlation coefficients
were estimated both in callus induction and growth characters. Five characters e.g. line callus
growth rate, relative fresh weight growth, relative growth rate and callus growth index were
included in the function, 7 pairs of combinations showed significant and positive correlation,
particularly callus growth index showed strong association with other three characters. Likewise,
five characters related to embryogenic properties were drawn into correlation analysis. Green
spotting was treated as successful completion of embryo culture. Green spotting was positively
and significantly correlate with number of embryos inoculated (0.826**) and number of calli
(0.796**). It is stated that BARI Gom-25 produced the highest green shoot with 83%, which was
followed by BARI gom-29 with 71%. Finally it may conclude that embryo culture protocol may
successfully launch to obtain distantly related hybrids to study cytology as well as breeding
values of different wheat genotypes. However, the developed wheat varieties of BARI in general
have high totipotency to regenerate new seedling derive from immature embryos. After
completion of investigation it may suggest that interspecific and intergeneric hybridization may
be included in advanced breeding programs of wheat. | en_US |
