| dc.description.abstract | In 2013 according to International Diabetic Federation of Bangladesh (IDF) the
prevalence of diabetes in Bangladesh was 6.3%, in 2017 6.9%, but some studies
estimated 8.5% to 10%. Diabetes prevalence have also increased in the northern part of
Bangladesh such as in Dinajpur. Till now no proper study have been conducted about the
prevalence of diabetes with proper biochemical analysis in Dinajpur district. The reason
for the increase of prevalence of diabetes mellitus is primarily due to smoking, sedentary
lifestyle, excess carbohydrate intake, obesity, food and water toxicity and finally heavy
metal deposition. The heavy metal(arsenic, cadmium, lead etc.) accumulation in the body
can eventually affect pancreatic function which in turn can cause insulin resistance or
halt insulin production leading to diabetes. Lactic acid bacteria can reduce the heavy
metal content of food and water and therefore can be an exact solution to this problem.
We have concluded in this experiment that fermented food such as rice can reduce the
amount of heavy metal content by a sufficient margin. Lactic acid bacteria have been
isolated from fermented rice and identified using 16s rDNA sequencing. A total of 1972
patients from various sub-districts of Dinajpur have been tested for blood glucose,
pancreatic amylase dysfunction, kidney dysfunction, Liver dysfunction etc. Out of all the
patients, 317 (16%) patients have been found to have diabetes, 19% patients have been
found with pancreatic amylase dysfunction, 56 (7.4%) patients were affected with kidney
disease and 19 (15.2%) have been found with liver disease. Meanwhile six different
lactic acid bacteria have been isolated from fermented rice sample and pure colony have
been grown on De Man, Rogosa and Sharpe agar (MRS) agar media. The 16s rDNA
gene sequencing showed 99% homology with Streptococcus sp. The isolate strains
Streptococcus sp. HSTU-1, Streptococcus sp. HSTU-3, Streptococcus sp. HSTU-4,
Streptococcus sp. HSTU-6, Streptococcus sp. HSTU-7 and Streptococcus sp. HSTU-8
have been identified and submitted to National Centre for Biotechnology Information
(NCBI) with accession number MK100837, MK100838, MK100839, MK100840,
MK100841, MK100842 respectively. The strains were subjected to biochemical
analysis. This study showed anine month data of diabetes patients screening from
different sub-districts of Dinajpur. About 19% of the patient have been found with
pancreatic abnormalities which is correlated with their food habit including more than
sufficient amount of rice in their diet. This proves that pancreatic abnormalities or
dysfunction happens due to heavy metal (arsenic, cadmium, lead) toxicity from water,
rice etc. Since the fermented rice revealed a diversity of Streptococcus sp. which has
been reported in various articles to reduce the amount of heavy metal from food, we can
conclude that by taking fermented rice samples the risk of diabetes or pancreatic
dysfunction can be reduced by a sufficient margin. And by doing so, the prevalence of
diabetes can substantially decrease. | en_US |
