| dc.description.abstract | Salmonellosis and Shigellosis bear the potential threat for human health. Outbreaks of
Salmonellosis and Shigellosis remain serious health problems worldwide. Therefore, the
present study was conducted to isolate and identify Salmonella and Shigella species from
stool samples of human patients. A total of 33 samples were collected from the Dinajpur
General Hospital in Bangladesh and brought to the laboratory of Microbiology, Faculty
of Veterinary and animal Science, Hajee Mohammad Danesh Science and Technology
University, Dinajpur. This study was performed by morphological, cultural, biochemical
test, serological test. In addition, antibiotic sensitivity was evaluated by disc diffusion
method during the period from January 2020 to June 2020.
The present study was obtained that the percentage of positive cases of Salmonella was
52.7% while the percentage of positive cases of Shigella was 28.6%. The study revealed
that the prevalence of Salmonella and Shigella isolated from human stool samples related
to male and female was (57.1% and 44.4%) respectively. The current study indicated that
the male was higher prevalence than the female. Out of 17 patients 6 were positive and
others were negative according to their age, the age of these participants were ranged 5-
15 years found (28.5%) (n=2) and (14.2%) (n=1) and 16-45 years of age obtained of
result (30%) (n=3) and (0.0%) (n=0) was found positive in Salmonella and Shigella
respectively. Also the overall prevalence related to age was (42.7% and 30%) of
Salmonella and Shigella respectively. Salmonella species isolated, out of 33 patients
were susceptible to Gentamicin, Cefixime and Chloramphenicol antibiotics. The
remaining patients displayed resistant to Cefoxitin, Erythromycin, Azithromycin,
Amoxicillin, Tetracycline and Ampicillin while intermediate to Levofloxacin. On the
other hand, Shigella species were sensitive to Gentamicin, Azithromycin and
Chloramphenicol. The isolates were resistant to Cefoxitin, Erythromycin, Levofloxacin,
Amoxicillin, Tetracycline and Ampicillin while intermediate to Cefixime. The present
study showed that the prevalence of Salmonella typhi, Salmonella paratyphi A and
Salmonella paratyphi B using qualitative slide agglutination reaction results of Widal test
was 39.4%, 12.1% and 6.1%, respectively. | en_US |
