PREGNANCY DIAGNOSIS OF SHEEP, GOATS AND CATTLE BY BARIUM CHLORIDE AND SEED GERMINATION INHIBITION TEST AT FIELD CONDITION

Abstract

Twenty one days non-returned cows and heifer (n=40), sheep (n=20) and goats (n=20) of pirganj and biral upazillas of Rangpur and Dinajpur Districts, respectively, were used to diagnose pregnancy using seed germination inhibition and barium chloride test. Animals were randomly divided into three groups. No germination with the change of colour having shoots length less than 1 cm were considered Pregnant. Germination 35-60% with moderated shoot length (4 cm), indicated non-pregnant. In controlled petri-dish, 60-80% germination with shoot length of about 6 cm were considered non-pregnant can go to dissimilar. The percentage of pregnant ewes diagnosed by seed germination inhibition test during 21-150 days of pregnancy were ranged from 21-35 days 75% (3/4) and 35-60 days 80% (4/5) of pregnancy results respectively. In goats pregnancy were ranged from 21-35 days 80% (4/5) and 35-60 days 87.5% (7/8) of pregnancy results respectively. The cows at 21-35 days pregnancy showed negative result. It was revealed that percentage of pregnant ewes diagnosed by barium chloride test during 21-150 days of pregnancy ranged from 21-35 days 75% (3/4) and 35-60 days 80% (4/5) of pregnancy results respectively. The goats ranged from 21-35 days 80% (4/5) and 35-60 days 87.5% (7/8) of pregnancy results respectively. The cows of 21-35 days pregnancy showed negative result. Seed germination inhibition test and Barium chloride test from 35 days of pregnancy in cow, the accuracy of pregnancy diagnosis was 90% (9/10). Hundred percent positive results were obtained from day 60-240 of pregnancy in cows. The total accuracy of pregnancy diagnosis was 90-100% from the day 35 to 240 by seed germination inhibition test and barium chloride test in cows