Anti- Diabetogenic Impact of Mahogany (Swietenia macrophylla) and Bitter melon (Momordica charantia) on Alloxan Induced Diabetic Rabbit Model

Abstract

The present study was undertaken to investigate the anti-diabetogenic effect of bitter melon and mahogany on alloxan induced diabetes in experimental rabbits. The Newzeland white breed male rabbit at the age of 4 months were randomly assigned into five groups (T1, T2, T3, T4 and T5) and each group was remind 4 rabbits. Group T1 was kept for negative control or normal group. The rest of the group (T2, T3, T4 and T5) were injected with alloxan intramuscularly @ 75mg/kg body weight of rabbit. So group T2 was positive control group for diabetes. Group T3, T4 and T5 was considered for bitter melon, mahogany and both of them at a dose of 250 mg /kg body weight, 50 mg/kg body weight and at with combined with previous dose and was considered T3, T4 and T5 respectively. After acclimatization, diabetes was induced in four groups of rabbits (T2, T3, T4 and T5) by administering alloxan injection in a dose of 75mg/kg body weight intramuscularly. The suspension of whole fruit was tested for its efficacy in alloxan induced diabetic rabbits. Over the course of the trial, observations were recorded for induction of diabetics, blood glucose level, body weight after 72 hours. Blood glucose level were increased significantly (p<0.05) in all treated groups compared to the control group and the highest induction was recorded in group T2 treated with alloxan. Body weight was decreased significantly (p<0.05) in alloxan treated group and lowest was recorded in group T2 which received alloxan treatment. There was significant decreased in in blood glucose level in all bitter melon and mahogany treated group (T3, T4, T5) compared to the T2 group and lowest glucose level was recorded in T5 group when treated with medicinal herbs and body weight was increased in all treated group T3, T4, T5 compare to the T2 group and highest was recorded in T5 group while treated with those. The present study reveals that combined treatment with bitter melon and mahogany increase body weight and decrease glucose level without affecting health of rabbits. The results of this study show that chronic oral administration of a suspension of mahogany seed and bitter melon fruits, an appropriate dosage may be good alternative as anti-diabetic agent.