| dc.description.abstract | The research work was under taken with a view to isolate and identify the etiological
agent from recent outbreaks of fowl cholera in layer chickens with antibiogram study
of identified field isolate. The entire research work was conducted in the Bacteriology
Laboratory of the Department of Microbiology, Hajee Mohammad Danesh Science
and Technology University (HSTU), Dinajpur. The samples were collected
aseptically and brought to the respective laboratory for proper isolation and
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identification by cultural, morphological and biochemical examinations. For this
purpose, a total of 96 samples (liver, spleen, heart) were collected from suspected live
(46 samples) and dead layer chickens (50 samples) from different areas of Dinajpur
district. From these 96 samples, 25 (42%) were found to be positive for Pateurella
multocida in live sick birds and 11 (24%) in dead birds. The isolated organism from
chickens produced whitish, opaque, round, flat, translucent colonies of sticky mucoid
or dry consistency about 1-3 mm in diameter on Nutrient Agar and whitish, opaque,
round, translucent colonies without hemolysis on Blood Agar and were able to
ferment dextrose, mannitol and sucrose but unable to ferment lactose and maltose on
biochemical examinations. The field isolates were identified by observing colony
morphology in different culture media, presence of rod shaped, bipolar Gram (-ve)
organism in Grams staining technique and different biochemical reaction. The
identified isolate were subjected to antibiogram study. In this study, it was observed
that the field isolate were highly sensitive to Ciprofloxacin, Levofloxacin and
Neomycin respectively. On the other side, the organisms were resistant to
Amoxycillin, Tetracycline and Bacitracin respectively. It was evident that
Ciprofloxacin, Levofloxacin and Clindamycin could be of better choice for the
treatment of fowl cholera in layer chickens. | en_US |
