POTENTIAL ANTIBIOTIC RESIDUAL RISK FROM BROILERS OF WET MARKETS IN MYMENSINGH SADAR

Abstract

This study was undertaken to investigate antibiotic residue status of broiler in live broiler market in Mymensingh Sadar. Altogether twenty live poultry selling shops were randomly selected for questionnaire study and sample collection. The sellers were asked different questions about antibiotic related knowledge, attitude, and practices. A pre-structured questionnaire was used to interview the participants. From each shop, two broilers were randomly selected for sample collection. They were sacrificed by halal method and liver and breast muscle were collected in individual zipper polythene bag. Fresh samples were processed for Thin Layer Chromatography (TLC) to detect antibiotic residue. All sellers were male and the number of participants were highest in 21-30 age group while the proportion of different age group varied significantly (P<0.01). Among them, 60% sellers had primary education followed by 25% secondary, and 5% higher secondary. Only 10% of the broiler sellers had no education. All sellers were familiar with antibiotics and vitamins. Besides, 57.5% and 22.5% of the broiler sellers had knowledge about growth promoter and withdrawal period respectively but no one had any knowledge regarding antibiotic residue. In addition, only 7.5% sellers understood the term antibiotic resistance and 12% had some knowledge on human health hazard of antibiotics misuse. Furthermore, all broiler sellers provided only commercial feed and water throughout entire selling period whereas 77.5% and 17.5% of them mixed vitamins and antibiotics in water to prevent any unwanted bird mortality. In purchasing of antibiotics, 32.5% of the sellers depended on feed sellers and only 20% of them used prescription provided by the registered veterinarian. The non-response rate was 47.5% in this case. The highest positive samples were found in Swadeshi bazar which was 41.6% followed by Shankipara bazar and Pouroshova bazar (33.3%), Notun bazar (25%), and Kachijhuli bazar (16.7%). The results obtained from different areas differed significantly (P<0.01).Fluoroquinolones group of antibiotics were most prevalent in the positive samples. Enrofloxacin positive sample was 50% followed by ciprofloxacin 27.8% and amoxicillin 22.2%. The results differed significantly (P<0.01). Among the positive samples, 40% was found in liver and 20% in breast muscle. The percentages didn‘t differ significantly (P<0.01). This present study revealed some valuable information regarding different antibiotic related knowledge, x attitude, and practice of live broiler sellers in wet market of Mymensingh sadar. It was observed that their lack of knowledge make the customers vulnerable to different health hazards. So, proper training and awareness building program regarding antibiotics use, misuse, and abuse might come handy in confronting the present antibiotic residue phenomena. In addition, the higher antibiotic residual percentage in different live broiler markets is a serious indication of harmful consequences for both poultry and human health.