CLINICAL DIAGNOSIS OF BOVINE DERMATOPHILOSIS WITH ANTIBIOGRAM IN DINAJPUR SADAR UPAZILLA

Abstract

Dermatophilosis is an economically important disease prevalent in Bangladesh. The study on clinical diagnosis of bovine dermatophilosis with antibiogram was undertaken to determine the epidemiological incidence based on season, age, sex, breed, concurrent infection and management system and mortality of the diseases. Clinical signs, therapeutic efficiency and microbiological isolation of the organism of the disease and their antibiotic sensitivity was also performed. The study were conducted at Upazilla veterinary hospital of Dinajpur Sadar from January–December, 2016. A total number of 320 cattle were physically observed and recorded among which 48 cattle were diagnosed and recorded for dermatophilosis infection. Very close visual inspection and palpation of the entire skin surface of the body were the clinical method for examination of animals. Crusts, skin scraping, plucked hair from the affected live animals were collected aseptically and transferred to microbiology laboratory of HSTU, Dinajpur for isolation and identification of the bacteria. The affected individuals were treated with three different types of therapeutics, group A treated with penicillin injection, group B with combination of penicillin and streptomycin and thirdly the group C were treated with long acting oxytetracycline. Collected data based on different epidemiological parameters were analyzed statistically. The highest incidence of bovine dermatophilosis were in summer season (18.33%) followed by `rainy season (5.45%) and winter season (4.0%). The annual incidence of dermatophilosis were highest in calf of more than 1 month to 1 year (20.83%), in male animal (15.85%), in rural housed hold farm (14.55%) and in indigenous cattle (16.21%). Management condition and season had highly significant (p<0.05) influence on the bovine dermatophilosis but age, sex and breed of cattle had no significant effect on the prevalence of dermatophilosis. The investigation recorded both acute and chronic from of the disease. Death of one calf due to generalized skin lesions all over the body was also seen. Isolation of bacteria on the blood agar was difficult in many cases; it was contaminated by unexpected secondary bacterial growth. Finally concluded, it was done successfully and the cultural characteristics were round to irregular, grayish white colonies and ȕ –hemolysis. In Gram‟s stain, the organism was gram positive, branching filamentous, rod and non-spore forming. In antibiotic sensitivity test the isolated organism inoculated in antibiotic disc where it produced clear circular zone and showed positive reaction to streptomycin followed by penicillin and gentamycin. The organism showed resistance to neomycin and kanamycin antibiotic.