ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF PASTEURELLA MULTOCIDA FROM CHICKENS AT DINAJPUR DISTRICT OF BANGLADESH

Abstract

The present research work was performed for the isolation and characterization of Pasteurella multocida from recent outbreak of chickens and demonstration of antibiotic sensitivity of the selected isolates. The entire research work was conducted primarily in the Bacteriology laboratory of the Department of Microbiology, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur. The samples were aseptically carried out to the laboratory and were subjected to different cultural, morphological and biochemical examinations. A number total of 106 samples inluding liver, spleen and heart were collected from suspected sick chickens (60 samples) and suspected dead chickens (46 samples) from different selected areas of Dinajpur district. From these samples, 25 (42 %) from suspected sick chickens and 11 (24 %) from suspected dead chickens were found to be positive for Pasteurella multocida. The isolated organism from chickens produced whitish, opaque, round, flat, translucent colonies of sticky mucoid or dry consistency about 1-3 mm in diameter on Nutrient Agar and whitish, opaque, round, translucent colonies without hemolysis on Blood Agar and were able to ferment dextrose, mannitol and sucrose but unable to ferment lactose and maltose on biochemical examinations. Form the antibiogram study, it was revealed that among the isolated Pateurella multocida from chicken, 100% were highly sensitive to Ciprofloxacin and Levofloxacin. 83% were to Neomycin, 78% were to Clindamycin and 3% were to Gentamycin. 75% were moderately sensitive to Doxycicline, 61% were to Gentamycin, 22% were to Clindamycin, 17% were to Neomycin and 14% were to Erythromycin. On the other hand 61% were less sensitive to Erythromycin; 17% were to Tetracycline; 13% were to Gentamicin, 25% were to Doxycicline and 8% were to Bacitracin. 100% isolates were resistant to Amoxycillin, 83% were to Tetracycline; 92% were to Bacitracin, and 25% were to Erythromycin.