IN VITRO PLANT REGENERATION OF CHILLI (Capsicum spp.) FROM LEAF EXPLANT

Abstract

A study was conducted with leaf explants of chilli for regeneration on MS medium supplemented with different concentrations and combination of auxins and cytokinins. For callus induction, five different concentrations and combinations of growth regulators viz. MS + 2.0 mg/L Kinetin +3.0 mg/L 2,4-D, MS+ 1.5 mg/L Kinetin +2.5 mg/L 2,4-D, MS+1.0 mg/L Kinetin +2.0 mg/L 2,4-D, MS+ 0.5 mg/L Kinetint+ 1.5 mg/L 2,4-D and MS +0.05 mg/L Kinetin +1.0 mg/L 2,4-D were used. The highest percentage of callus( 91.67%) was obtained in MS medium supplemented with 2.0mg/L Kinetin + 3.0 mg/L 2,4-D 7.00 days. Among the three varieties, Siam hot showed the best performance on callus induction. On the other hand, leaf induced shoot bud in different combinations of MS medium supplemented with different combinations like MS+3.0 mg/L IAA+ 1.0 mg/L BAP, MS+ 3.0 mg/L IAA +2.0 mg/L BAP,MS + 1.5 mg/L IAA +2.5 mg/L BAP,MS +1.0 mg/L IAA +3.5 mg/L BAP and MS+ 1.0 mg/L IAA+ 5.0 mg/L BAP. Leaf explants showed the best performance in MS+3.0 mg/L IAA+1.0 mg/L BAP in terms of percent shoot regeneration(71.67%) and number of callus showing shoot (14.33). The shoot bud showed the best performance in MS+ 3.0 mg/L IAA+1.0 mg/L BAP combination and Siam hot showed the remarkable performance on shoot regeneration. On the other hand, best root regeneration (80.00%) was found on MS+3.0 mg/L IBA+1.0 mg/L BAP combination and the genotype Siam hot showed the best performance on root regeneration.