THROMBOLYTIC AND BLEEDING TIME PROPERTIES OF ETHANOLIC EXTRACT OF Carum copticum AND Nicotiana tabbacum

Abstract

In this present study, the leaves extracts of tobacco leaves and azwain subjected to the thrombolytic activities were assessed by using human erythrocyte and the results were compared with standard streptokinase (SK). They have great impact on bleeding time and clotting time. An experiment was carried out in the laboratory of the department of physiology and pharmacology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, during the period of JulyDecember/2014. Some plant and spices were collected from Dinajpur district. Primarily ethanol extract was prepared from those plant and spices. 20 micropipette was taken filled with 0.5 ml human blood and 3 micropipette was taken as excess micropipette for control. These two kind of micropipette were screened for assessment their comparative efficacy of clotted blood lysis. Ethanol extract was used at a dose of 100 micro ml/ micropipette. Then this micropipette was incubated at 37 degree centigrade temperature for 1hr 45 minutes. After incubation I found 4 plant and 2 spices has anticoagulant property. These plant are tobacco flower, Fern, Arum, Cannabis Tezpata and spices are Jowin and Pesta. Finally I was experimented with Jowin and tobacco in-vivo thrombolytic and bleed time property .Bleed time and thrombolytic property was experimented in laboratory compared with Streptokinase (SK). Streptokinase showed 88.13% efficacy after treatment on rabbit. Efficacy was recorded by compare among streptokinase control treated extract. Ethanol jawin (Carum copticum) extract lysis cloted blood 53.24% and ethanolic tobacco (Nicotiana tabacum) flower extract lysis cloted blood 46.28%.